Show simple item record Maron, John L. Johnson, Marc T. J. Hastings, Amy P. Agrawal, Anurag A.
dc.coverage.spatial Northeastern USA
dc.coverage.temporal modern 2017-12-07T22:13:02Z 2017-12-07T22:13:02Z 2017-12-05
dc.identifier doi:10.5061/dryad.bn37p
dc.identifier.citation Maron JL, Johnson MT, Hastings AP, Agrawal AA (2017) Fitness consequences of occasional outcrossing in a functionally asexual plant (Oenothera biennis ). Ecology, online in advance of print.
dc.description Many clonal organisms occasionally outcross, but the long-term consequences of such infrequent events are often unknown. During five years, representing three to five plant generations, we followed 16 experimental field populations of the forb, Oenothera biennis, originally planted with the same 18 original genotypes. Oenothera biennis usually self-fertilizes, which due to its genetic system (Permanent Translocation Heterozygosity), results in seeds that are clones of the maternal plant. However, rare outcrossing produces genetically novel offspring (but without recombination or increased heterozygosity). We sought to understand whether novel genotypes produced through natural outcrossing had greater fecundity or different multigenerational dynamics compared to our original genotypes. We further assessed whether any differences in fitness or abundances through time between original and novel genotypes were exaggerated in the presence versus absence of insect herbivores. Over the course of the experiment, we genotyped >12,500 plants using microsatellite DNA markers to identify and track the frequency of specific genotypes and estimated fecundity on a subset (>3000) of plants. The effective outcrossing rate was 7.3% in the first year and ultimately 50% of the plants were of outcrossed origin by the final year of the experiment. Lifetime fruit production per plant was on average 32% higher across all novel genotypes produced via outcrossing compared to the original genotypes, and this fecundity advantage was significantly enhanced in populations lacking herbivores. Among 43 novel genotypes that were abundant enough to phenotype with replication, plants produced nearly 30% more fruits than the average of their specific two parental genotypes, and marginally more fruits (8%) than their most fecund parent. Mean per capita fecundity of novel genotypes predicted their relative frequencies at the end of the experiment. Novel genotypes increased more dramatically in herbivore-present compared to suppressed populations (45% vs. 27% of all plants), countering the increased competition from dandelions (Taraxacum officinale) that resulted from herbivore suppression. Increased interspecific competition likely also lead to the lower realized fitness of novel versus original genotypes in herbivore-suppressed populations. These results demonstrate that rare outcrossing and the generation of novel genotypes can create high-fecundity progeny, with the biotic environment influencing the dynamical outcome of such advantages.
dc.relation.haspart doi:10.5061/dryad.bn37p/1
dc.relation.haspart doi:10.5061/dryad.bn37p/2
dc.relation.isreferencedby doi:10.1002/ecy.2099
dc.subject adaptation
dc.subject outcrossing
dc.subject Permanent translocation heterozygote
dc.subject rapid evolution
dc.title Data from: Fitness consequences of occasional outcrossing in a functionally asexual plant (Oenothera biennis)
dc.type Article
dwc.ScientificName Oenothera biennis
dc.contributor.correspondingAuthor Maron, John
prism.publicationName Ecology

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Title fecundity data 2008-2012
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Description Fecundity data for all Oenother biennis plants that were genotyped in each plot each year 2008-2012.
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Title frequency of genotypes
Downloaded 3 times
Description Frequency data for the original 18 genotypes and the 8 most common outcrossed genotypes by year and plot. Frequencies sum to 1 as rare genotypes are excluded.
Download frequency of genotypes.csv (18.23 Kb)
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