Data from: Investigating a possible role for the bacterial signal molecules N-acylhomoserine lactones in Balanus improvisus cyprid settlement

 

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Title Tait and Havenhand Biofilm data
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Description Biofilms data.txt: This data records the number of Balanus improvisus cyprids exploring (Day 2 data only) and the number of cyprids settled (Days 3 to 7 data) on biofilms of signal-producing and signal-deficient biofilms. Biofilms either had mutations to their AHL synthase gene or were expressing the recombinant AHL inactivation protein AiiA. The biofilms were: Vibrio anguillarum NB10, a vanM mutant of this strain, and a variant expressing aiiA; A. hydrophila and an ahylI mutant and Sulfitobacter sp. BR1 and a sulI mutant. Each experiment also contained no biofilm controls. Experiments with V. anguillarum were conducted with 3 batches of cyprids, Sulfitobacter BR1 with 2 batches of cyprids and A. hydrophila with 1 batch of cyprids. This data was recorded by Karen Tait, Plymouth Marine Laboratory (ktait@pml.ac.uk) at the Department of Biological and Environmental Sciences, Tjarno, Sweden in June 2009. Column headings: V. anguillarum Batch 1; V. anguillarum Batch 2; V. anguillarum Batch 3; A. hydrophila Batch 1; Sulfitobacter Batch 1; Sulfitobacter Batch 2. Sub-headings: Biofilm Type (WT, mutant or no biofilm); Vessel No.; Day 2 (percent number of cyprids actively exploring biofilm); Day 3 (percent number of cyprids settled); Day 4 (percent number of cyprids settled); Day 5 (percent number of cyprids settled); Day 6 (percent number of cyprids settled); Day 7 (percent number of cyprids settled). Mutant biofilm descriptions are as follows: vanM – V. anguillarum, mutation to the AHL synthase, vanM; aiiA – V. anguillarum expressing the AHL degradation protein AiiA; ahyI – A. hydrophila, mutation to the AHL synthase, ahyl; sulI – Sulfitobacter BR1, mutation to the AHL synthase, sulI.
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Title Tait and Havenhand E. coli data
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Description E. coli data.txt: This data records the number of Balanus improvisus cyprids exploring biofilms of E. coli expressing recombinant AHL synthases after 2 days incubation. For each AHL synthase gene, this is compared to a background control (E. coli carrying the same plasmid without the AHL synthase insertion). Data is the percent number of cyprids actively exploring (crawling) on the biofilms. This data was recorded by Karen Tait, Plymouth Marine Laboratory (ktait@pml.ac.uk) at the Department of Biological and Environmental Sciences, Tjarno, Sweden in June 2009. Column headings: Vessel number (numbered 1 – 21); no biofilm (vessel contained no biofilm); E. coli pGEM (control biofilm); E. coli pGEM sulI (produces the AHLs C4-HSL, HC6-HSL, C8-HSL and OC10-HSL); E. coli pT3T7 (control biofilm); E. coli pT3T7 luxI (produces the AHL OC6-HSL); E. coli pUCP18 (control biofilm); E. coli pUCP18 rhlI (produces the AHL C4-HSL); E. coli pET3a (control biofilm); E. coli pET3a vanI (produces the AHL OC10-HSL).
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Title Tait and Havenhand Synthetic AHL data
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Description Synthetic AHL data.txt: This data records the number of Balanus improvisus cyprids exploring agarose films containing 5 µM C6-HSL, C8-HSL, OC10-HSL and C12-HSL after 2 days incubation. Data is the percent number of cyprids actively exploring (crawling) on the biofilms. Also recorded is the numbers of cyprids settled within vessels containing the same synthetic AHLs dissolved in seawater to the following concentrations: 0.5, 5 and 50 µM. Data is the percent number of cyprids settled within the vessels. This data was recorded by Karen Tait, Plymouth Marine Laboratory (ktait@pml.ac.uk) at the Department of Biological and Environmental Sciences, Tjarno, Sweden in June 2009. Column headings: Synthetic AHL in agarose film; Vessel number (numbered 1 – 18); C6; C8; OC10; C12; no AHL. Synthetic AHL in seawater; AHL concentration (0, 0.5, 5 or 50 µM); Vessel number (numbered 1 – 21 for each treatment); C6; C8; OC10; C12.
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When using this data, please cite the original publication:

Tait K, Havenhand J (2013) Investigating a possible role for the bacterial signal molecules N-acylhomoserine lactones in Balanus improvisus cyprid settlement. Molecular Ecology 22(9): 2588–2602. http://dx.doi.org/10.1111/mec.12273

Additionally, please cite the Dryad data package:

Tait K, Havenhand J (2013) Data from: Investigating a possible role for the bacterial signal molecules N-acylhomoserine lactones in Balanus improvisus cyprid settlement. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.c3b75
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