Data from: Temporal variation in antibiotic environments slows down resistance evolution in pathogenic Pseudomonas aeruginosa

Roemhild R, Barbosa C, Beardmore RE, Jansen G, Schulenburg H

Date Published: October 13, 2015

DOI: http://dx.doi.org/10.5061/dryad.c867b.2

 

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Title Data for dose response curves
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Description This file contains the data for the dose-response curves as shown in supplementary Fig. S2 of the paper. The columns indicate the following: $date: the data was collected on two days; $treatment: specifies antibiotic treatment; $ID: a number from "0" to "10" that serves as ID for the ascending antibiotic concentrations; $conc_ng_ml: concentration of antibiotic in ng/ml; $replicate: name of technical replicate; $od_600: optical density at 600 nm read after 12h of incubation at 37°C.
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Title Data for both evolution experiments
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Description This file contains the data from the two evolution experiments, as summarized in Figs. 1,2,3,5,S3,S5, and S6 of the paper. The columns show the following: $well: position of population in 96-well plate; $season: number of transfers within the evolution experiment; $time: time point of od measurement in hours; od was measured in 15 min intervals; $od_600: optical density at 600 nm; $ID: a number from "1" to "10" that serves as ID for treatment groups as specified in the paper plus "11" for the evolving nodrug references; $treatment: treatment type, including monotherapy (mono), regular changes of antibiotics (regular), and random changes of antibiotics (random); $drug: antibiotic used; $exp: number of evolution experiment.
Download Data2_Roemhild_2015a.csv (6.834 Mb)
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Title Data for cross-resistance test
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Description This file contains the data for the cross-resistance test (Fig. 4). $evoexp: number of evolution experiment or the ancestral population $evoexp_season: season of the evolution experiment after which populations were streaked on agar plates for characterization $evoexp_drug: antibiotic present in $evoexp_season and on the agar plates $evoexp_well: position of population in 96-well plate during evolution experiment $clone_id: name of the five cfus that were isolated from each $evoexp_well $test_drug: antibiotic present in the cross-resistance test $ic: the inhibitory concentration aimed for with the applied concentration; if the value is "E" these are wells with antibiotic but without the bacteria $concentration_ng_ml: concentration of the antibiotic in the cross-resistance test in ng/ml $od_600: optical density at 600 nm after 12h of incubation at 37°C
Download Datafile3_Roemhild_2015_Corrigendum.txt (42.84 Kb)
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When using this data, please cite the original publication:

Roemhild R, Barbosa C, Beardmore RE, Jansen G, Schulenburg H (2015) Temporal variation in antibiotic environments slows down resistance evolution in pathogenic Pseudomonas aeruginosa. Evolutionary Applications 8(10): 945-955. http://dx.doi.org/10.1111/eva.12330

Additionally, please cite the Dryad data package:

Roemhild R, Barbosa C, Beardmore RE, Jansen G, Schulenburg H (2015) Data from: Temporal variation in antibiotic environments slows down resistance evolution in pathogenic Pseudomonas aeruginosa. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.c867b.2
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