Data from: Experimental evolution of the Caenorhabditis elegans sex determination pathway

 

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Title Cel_EE_geneexpression_master
Downloaded 72 times
Description Contains gene expression data derived from qRT-PCR. See publication for methodological details. sample: The strain/line and biological replicate (e.g., 16EE1b is from strain 16EE1, replicate b; AB1 3 is from strain AB1, replicate 3). gene: The gene in which expression was assayed. plate: The reaction plate in which RT-PCR was performed; the experiment was performed on a total of five 96-well plates. ct: The threshold cycle (CT-value) for each sample. ctcontrol: The threshold cycle (CT-value) for reference gene using the same biological sample (template RNA) from the same reaction plate. deltact: The difference between the experimental and reference CT-values (experimental - reference)
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Title EEPhenotypeData
Downloaded 50 times
Description Contains tail scores for individual worms of each strain reared at six temperatures. See publication for methodological details. ID: ID number for individual worm. Strain: Strain/line of each worm. Temp: Temperature at which each worm was raised (degrees C). Tail: Tail score (see Chandler, 2010, Heredity, 105, 473-482 for scoring details).
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Title EEFitnessData
Downloaded 52 times
Description Contains tail scores and gonad phenotypes for individual worms of each strain reared at six temperatures. See publication for methodological details. ID: ID number for individual worm. Strain: Strain/line of each worm. Temp: Temperature at which each worm was raised (degrees C). Tail: Tail score (see Chandler, 2010, Heredity, 105, 473-482 for scoring details). Gonad: Gonad score for each worm, -1 = unscorable, 0 = hermaphrodite with obvious eggs, 1 = hermaphrodite carrying no visible eggs, 2 = looks like a hermaphrodite gonad, but abnormal with no eggs (categories 1 & 2 were pooled together for analyses in the publication), 3 = male.
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When using this data, please cite the original publication:

Chandler CH, Chadderdon GE, Phillips PC, Dworkin I, Janzen FJ (2011) Experimental evolution of the Caenorhabditis elegans sex determination pathway. Evolution 66(1): 82-93. http://dx.doi.org/10.1111/j.1558-5646.2011.01420.x

Additionally, please cite the Dryad data package:

Chandler CH, Chadderdon GE, Phillips PC, Dworkin I, Janzen FJ (2011) Data from: Experimental evolution of the Caenorhabditis elegans sex determination pathway. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.cg872
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