Data from: Cold adaptation increases rates of nutrient flow and metabolic plasticity during cold exposure in Drosophila melanogaster

Williams CM, McCue MD, Sunny NE, Szejner-Sigal A, Morgan TJ, Allison DB, Hahn DA

Date Published: August 17, 2016

DOI: http://dx.doi.org/10.5061/dryad.d04s0

 

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Title Experiment 1 – Quantifying anabolism from glucose in cold-hardy versus -susceptible fly lines
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Description Cold hardy and susceptible flies were fed a dilute U13C-6-glucose tracer, left to metabolize for 4h, then homogenized and enrichments in glucose and amino acids were analyzed using GC-MS as described in Williams et al. (in review). Data are Atom Percent Excess (APE).
Download Expt 1_glucose and AA synthesis_15Jun2016.xlsx (12.06 Kb)
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Title Experiments 2 and 3 - Oxidation rates of glucose and leucine tracers
Downloaded 1 time
Description Oxidation rates of glucose and leucine tracers before, during and after cold exposure for cold-hardy and -susceptible flies.
Download Expt2and3_Breathoxidationrates_15Jun2016.xlsx (23.35 Kb)
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Title Experiments 2 and 3 - Tracer enrichments in body pools
Downloaded 4 times
Description 13C enrichments in lipid, carbohydrate and protein pools resulting from glucose and leucine tracer ingestion, before during and after cold exposure for cold-hardy and cold-susceptible flies.
Download Expts2and3_body enrichments_15Jun2016.xlsx (36.02 Kb)
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When using this data, please cite the original publication:

Williams CM, McCue MD, Sunny NE, Szejner-Sigal A, Morgan TJ, Allison DB, Hahn DA (2016) Cold adaptation increases rates of nutrient flow and metabolic plasticity during cold exposure in Drosophila melanogaster. Proceedings of the Royal Society B 283(1838): 20161317. http://dx.doi.org/10.1098/rspb.2016.1317

Additionally, please cite the Dryad data package:

Williams CM, McCue MD, Sunny NE, Szejner-Sigal A, Morgan TJ, Allison DB, Hahn DA (2016) Data from: Cold adaptation increases rates of nutrient flow and metabolic plasticity during cold exposure in Drosophila melanogaster. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.d04s0
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