Data from: DNA barcoding and metabarcoding of standardized samples reveal patterns of marine benthic diversity

Leray M, Knowlton N

Date Published: February 5, 2015

DOI: http://dx.doi.org/10.5061/dryad.d0r79

 

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Title PGM_RAWDATA_file1
Downloaded 46 times
Description This file contains raw PGM (Ion Torrent, Life technology) data for adaptors 7, 8, 10 and 11. See file "PGM_SequencingRun" for corresponding samples.
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Title PGM_RAWDATA_file2
Downloaded 39 times
Description This file contains raw PGM (Ion Torrent, Life technology) data for adaptors 12, 13, 14 and 15. See file "PGM_SequencingRun" for corresponding samples.
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Title Metabarcode_OTU_table
Downloaded 67 times
Description The column "source ID" presents how each OTU was identified. SAP: Statistical assignment Package; NCBI/BOLD: >97% match to a reference barcode in NCBI and/or BOLD barcode databases; 2mm: match an OTU identified via DNA barcoding in the >2mm fraction; 500μm: match an OTU identified via DNA barcoding in the 2mm-500μm fraction The second sheet lists all OTUs that were discarded because they matched bacterial or non-COI sequences in NCBI.
Download Metabarcode_OTU_table.xls (1.125 Mb)
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Title PGM_SequencingRun
Downloaded 66 times
Description The table presents the strategy used for multiplexing samples. Full primer sequences are provided in Table S4
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Title Metabarcode_OTUrepresentatives
Downloaded 15 times
Description The file contains one representative sequence per OTU
Download Metabarcode_OTUrepresentatives.fasta (715.6 Kb)
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Title Metabarcode_discardedOTUrepresentatives
Downloaded 7 times
Description The file contains one representative sequence per discarded OTU
Download Metabarcode_discardedOTUrepresentatives.fasta (84.87 Kb)
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When using this data, please cite the original publication:

Leray M, Knowlton N (2015) DNA barcoding and metabarcoding of standardized samples reveal patterns of marine benthic diversity. Proceedings of the National Academy of Sciences of the United States of America 112(7): 2076-2081. http://dx.doi.org/10.1073/pnas.1424997112

Additionally, please cite the Dryad data package:

Leray M, Knowlton N (2015) Data from: DNA barcoding and metabarcoding of standardized samples reveal patterns of marine benthic diversity. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.d0r79
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