Data from: Identification of the Minimal Cytolytic Unit for Streptolysin S and an Expansion of the Toxin Family

Molloy EM, Casajens SR, Cox CL, Maxson T, Ethridge NA, Margos G, Fingerle V, Mitchell DA

Date Published: July 28, 2015

DOI: http://dx.doi.org/10.5061/dryad.d4863

 

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Title MLSA Borrelia ML tree
Downloaded 9 times
Description A tree that shows the relationships of a number of Borrelia strains, including a subset of the Bbsl strains included in our PCR screen. A plus sign (+) next to the strain designation indicates that gene(s) from the Bor TOMM biosynthetic cluster (borB/borC/borD) was/were detected. WGS: whole genome sequence.
Download MLST tree_Doug_subm.nwk (2.751 Kb)
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Title SLS like TOMM D alignment
Downloaded 7 times
Description This is the alignment file for the maximum-likelihood tree based on the D protein for the majority of known and putative producers of cytolytic TOMMs (as of December 2014).
Download SLS-Like TOMM D Alignment.txt (668.8 Kb)
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Title Borrelia TOMM Alignment
Downloaded 10 times
Description BorB (A), borC (B) and borD (C) from Borrelia afzelii PKo (Bafz), B. spielmanii A14S (Bspi), and B. valaisiana VS116 (Bval) were aligned using ClustalW. Sites where PCR screening primers annealed are underlined. The primers used to detect borB/C/D were based on bvalB/C/D from B. valaisiana VS116
Download BorreliaTOMMAlignment.docx (122.3 Kb)
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Title SLS like Precursor Alignment
Downloaded 12 times
Description Alignment using Clustal Omega [49] reveals that the SLS core region possesses a highly conserved N-terminus containing 9-10 contiguous heterocylizable residues (underlined), while the C-terminus is variable in terms of both identity of residues and length. The putative leader peptide cleavage sites are shown as carets. Residues of SagA from S. pyogenes are numbered.
Download SLSlikePrecursorAlignment.docx (42.71 Kb)
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Title SLS like TOMM D ML Tree
Downloaded 5 times
Description A maximum-likelihood tree based on the D protein for the majority of known and putative producers of cytolytic TOMMs (as of December 2014).
Download SLS-like TOMM D ML Tree.nwk (34.87 Kb)
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Title TOMM D Alignment
Downloaded 11 times
Description An alignment file for the maximum-likelihood tree of a representative sample of TOMMs based on the D protein from each cluster. Clades are color-coded based on the predicted class of TOMM natural product. NHLP, nitrile hydratase leader peptide; NHLP-Burk, NHLP from Burkholderia; PZN, plantazolicin; Balh, uncharacterized TOMM from Bacillus sp. Al Hakam; McB, microcin B from Gammaproteobacteria. SLS, streptolysin S; Bor TOMM, putative SLS-like cytolysin from Bbsl.
Download TOMM D Alignment (51.70 Kb)
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Title TOMM D ML Tree
Downloaded 7 times
Description A maximum-likelihood tree of a representative sample of TOMMs based on the D protein from each cluster. Clades are color-coded based on the predicted class of TOMM natural product. NHLP, nitrile hydratase leader peptide; NHLP-Burk, NHLP from Burkholderia; PZN, plantazolicin; Balh, uncharacterized TOMM from Bacillus sp. Al Hakam; McB, microcin B from Gammaproteobacteria. SLS, streptolysin S; Bor TOMM, putative SLS-like cytolysin from Bbsl.
Download TOMM D ML Tree.nwk (1.48 Kb)
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Title MLSA Borrelia ML tree alignment
Downloaded 6 times
Description This is the alignment file for the tree that shows the relationships of a number of Borrelia strains, including a subset of the Bbsl strains included in our PCR screen. A plus sign (+) next to the strain designation indicates that gene(s) from the Bor TOMM biosynthetic cluster (borB/borC/borD) was/were detected. WGS: whole genome sequence.
Download MLSABorreliaMLtree.txt (353.6 Kb)
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When using this data, please cite the original publication:

Molloy EM, Casajens SR, Cox CL, Maxson T, Ethridge NA, Margos G, Fingerle V, Mitchell DA (2015) Identification of the Minimal Cytolytic Unit for Streptolysin S and an Expansion of the Toxin Family. BMC Microbiology 15: 141. http://dx.doi.org/10.1186/s12866-015-0464-y

Additionally, please cite the Dryad data package:

Molloy EM, Casajens SR, Cox CL, Maxson T, Ethridge NA, Margos G, Fingerle V, Mitchell DA (2015) Data from: Identification of the Minimal Cytolytic Unit for Streptolysin S and an Expansion of the Toxin Family. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.d4863
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