Data from: Oligonucleotide primers for targeted amplification of single-copy nuclear genes in apocritan Hymenoptera

Hartig G, Peters RS, Borner J, Etzbauer C, Misof B, Niehuis O

Date Published: December 17, 2012

DOI: http://dx.doi.org/10.5061/dryad.d73k0

 

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Title Amplicons_NT.tar
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Description Supplementary File Archive: "Amplicons_NT.tar.gz." Multiple nucleotide sequence alignments in FASTA format of expected amplicons when applying the PCR oligonucleotide primer pairs inferred in our study. Each multiple nucleotide sequence alignment contains the nucleotide sequence of each of the nine species of Hymenoptera whose genomes we analyzed plus the position and nucleotide sequence (with IUPAC ambiguity code; complement sequence of reverse primer) of the inferred forward and reverse PCR oligonucleotide primer. Anatomy of the file names: [Identifier of ortholog group]_[Identifier of oligonucleotide primer pair specific to ortholog group]_[Identifier of recommended pair of sequencing primers].NT.fas For example, the file name "HOG2939_01_B.NT.fas" contains the nucleotide sequences expected to be amplified from the genes belonging to ortholog group "HOG2939" when using the first oligonucleotide primer pair that has been inferred for this ortholog group and the sequencing oligonucleotide primer pair B. HOG stands for "Hymenoptera Ortholog Group".
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When using this data, please cite the original publication:

Hartig G, Peters RS, Borner J, Etzbauer C, Misof B, Niehuis O (2012) Oligonucleotide primers for targeted amplification of single-copy nuclear genes in apocritan Hymenoptera. PLoS ONE 7(6): e39826. http://dx.doi.org/10.1371/journal.pone.0039826

Additionally, please cite the Dryad data package:

Hartig G, Peters RS, Borner J, Etzbauer C, Misof B, Niehuis O (2012) Data from: Oligonucleotide primers for targeted amplification of single-copy nuclear genes in apocritan Hymenoptera. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.d73k0
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