Data from: The gut microbiota of the pine weevil is similar across Europe and resembles that of other conifer-feeding beetles

Berasategui A, Axelsson K, Nordlander G, Schmidt A, Borg-Karlson A, Gershenzon J, Terenius O, Kaltenpoth M

Date Published: May 28, 2016

DOI: http://dx.doi.org/10.5061/dryad.f3r67.2

 

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Title Quality filtered 454 sequence data
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Title Mapping file
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Description Mapping file for quality trimmed 454 sequences of bacterial 16s rRNA from the gut of Hylobius abietis
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Title OTU table generated after closed reference OTU picking strategy
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Title OTU table generated after open reference OTU picking strategy
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Title Phylogenetic tree of bacterial sequences from different beetles feeding on coniferous and non coniferous food sources
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Description The phylogeny was reconstructed with FastTree using the GTR model.
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Title Alignment_for_meta-analysis
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Description Our data set on H. abietis-associated bacteria was expanded by the addition of 308 bacterial sequences from GenBank associated with ten different insect host species belonging to four genera. These sequences included bacterial taxa found in the gut microbiota of five species of bark beetles belonging to two genera that feed on conifers, as well as five weevil species belonging to two genera that feed on red palm trees and ornamental plants, respectively . We only selected culture-independent studies. Database sequences were aligned with those obtained in this study using SINA. The alignment was imported into ARB together with the SILVA 115 database and a phylogenetic tree was constructed. We refined the alignment by selecting all H.abietis-associated sequences and those of closely related bacterial type strains present in the SILVA database. The final alignment was produced by filtering some sequences: we only retained those sequences from the pine weevil that corresponded to the 50 most prevalent OTUs and in addition those of minor OTUs that clustered together with other bark beetle or weevil-associated bacterial sequences. We also eliminated those bacterial sequences from bark beetles and weevils (other than H. abietis) that did not cluster together with any other insect-associated bacteria in this study.
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Title Full raw 454 sequencing fasta file
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Title Full raw 454 sequencing quality file
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When using this data, please cite the original publication:

Berasategui A, Axelsson K, Nordlander G, Schmidt A, Borg-Karlson A, Gershenzon J, Terenius O, Kaltenpoth M (2016) The gut microbiota of the pine weevil is similar across Europe and resembles that of other conifer-feeding beetles. Molecular Ecology, online in advance of print. http://dx.doi.org/10.1111/mec.13702

Additionally, please cite the Dryad data package:

Berasategui A, Axelsson K, Nordlander G, Schmidt A, Borg-Karlson A, Gershenzon J, Terenius O, Kaltenpoth M (2016) Data from: The gut microbiota of the pine weevil is similar across Europe and resembles that of other conifer-feeding beetles. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.f3r67.2
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