Data from: A from-benchtop-to-desktop workflow for validating HTS data and for taxonomic identification in diet metabarcoding studies

Corse E, Meglécz E, Archambaud G, Ardisson M, Martin J, Tougard C, Chappaz R, Dubut V

Date Published: August 2, 2017

DOI: http://dx.doi.org/10.5061/dryad.f40v5

 

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Title COI-filtering-DB.fas.tar
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Description Custom COI database used during HTS filtering in order to keep only relevant COI sequences
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Title Taxassign-DB.fas.tar
Downloaded 3 times
Description Custom COI database used for taxonomic assignement. It contains selected COI sequences from BOLD and sequences produced in the lab (MF458551 - MF458851). Sequences are in fasta format. ID lines contain taxon names and NCBI TaxIDs. If NCBI TaxID is not available for the taxon, the TaxID of its lowest level parent is given.
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Title Perl_scripts.tar
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Description Perl script used for data filtering and taxonomic assignment.
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Title MFZR1_S4_L001_R1_001.fastq.tar
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Description Fastq file with raw data. Forward sequences of the first replicate series amplified by MFZR primer pairs. Tag combinations to identify the samples are found in the associated readme file.
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Download MFZR1_S4_L001_R1_001.fastq.tar.gz (124.5 Mb)
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Title MFZR1_S4_L001_R2_001.fastq.tar
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Description Fastq file with raw data. Reverse sequences of the first replicate series amplified by MFZR primer pairs. Tag combinations to identify the samples are found in the associated readme file.
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Download MFZR1_S4_L001_R2_001.fastq.tar.gz (153.6 Mb)
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Title MFZR2_S5_L001_R1_001.fastq.tar
Downloaded 8 times
Description Fastq file with raw data. Forward sequences of the second replicate series amplified by MFZR primer pairs. Tag combinations to identify the samples are found in the associated readme file.
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Download MFZR2_S5_L001_R1_001.fastq.tar.gz (119.1 Mb)
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Title MFZR2_S5_L001_R2_001.fastq.tar
Downloaded 5 times
Description Fastq file with raw data. Reverse sequences of the second replicate series amplified by MFZR primer pairs. Tag combinations to identify the samples are found in the associated readme file.
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Title MFZR3_S6_L001_R1_001.fastq.tar
Downloaded 7 times
Description Fastq file with raw data. Forward sequences of the third replicate series amplified by MFZR primer pairs. Tag combinations to identify the samples are found in the associated readme file.
Download MFZR3_S6_L001_R1_001.fastq.tar.gz (141.8 Mb)
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Title MFZR3_S6_L001_R2_001.fastq.tar
Downloaded 5 times
Description Fastq file with raw data. Reverse sequences of the third replicate series amplified by MFZR primer pairs. Tag combinations to identify the samples are found in the associated readme file.
Download MFZR3_S6_L001_R2_001.fastq.tar.gz (168.2 Mb)
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Title ZFZR1_S1_L001_R1_001.fastq.tar
Downloaded 6 times
Description Fastq file with raw data. Forward sequences of the first replicate series amplified by ZFZR primer pairs. Tag combinations to identify the samples are found in the associated readme file.
Download ZFZR1_S1_L001_R1_001.fastq.tar.gz (130.0 Mb)
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Title ZFZR1_S1_L001_R2_001.fastq.tar
Downloaded 5 times
Description Fastq file with raw data. Reverse sequences of the first replicate series amplified by ZFZR primer pairs. Tag combinations to identify the samples are found in the associated readme file.
Download ZFZR1_S1_L001_R2_001.fastq.tar.gz (153.6 Mb)
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Title ZFZR2_S2_L001_R1_001.fastq.tar
Downloaded 6 times
Description Fastq file with raw data. Forward sequences of the second replicate series amplified by ZFZR primer pairs. Tag combinations to identify the samples are found in the associated readme file.
Download ZFZR2_S2_L001_R1_001.fastq.tar.gz (111.9 Mb)
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Title ZFZR2_S2_L001_R2_001.fastq.tar
Downloaded 6 times
Description Fastq file with raw data. Reverse sequences of the second replicate series amplified by ZFZR primer pairs. Tag combinations to identify the samples are found in the associated readme file.
Download ZFZR2_S2_L001_R2_001.fastq.tar.gz (133.1 Mb)
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Title ZFZR3_S3_L001_R1_001.fastq.tar
Downloaded 6 times
Description Fastq file with raw data. Forward sequences of the third replicate series amplified by ZFZR primer pairs. Tag combinations to identify the samples are found in the associated readme file.
Download ZFZR3_S3_L001_R1_001.fastq.tar.gz (128.6 Mb)
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Title ZFZR3_S3_L001_R2_001.fastq.tar
Downloaded 6 times
Description Fastq file with raw data. Reverse sequences of the third replicate series amplified by ZFZR primer pairs. Tag combinations to identify the samples are found in the associated readme file.
Download ZFZR3_S3_L001_R2_001.fastq.tar.gz (154.0 Mb)
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Title alignements_for_phylogeny.tar
Downloaded 3 times
Description Sequence alignments used for constructing phylogenetic trees of variants and their homologues.
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When using this data, please cite the original publication:

Corse E, Meglécz E, Archambaud G, Ardisson M, Martin J, Tougard C, Chappaz R, Dubut V (2017) A from-benchtop-to-desktop workflow for validating HTS data and for taxonomic identification in diet metabarcoding studies. Molecular Ecology Resources, online in advance of print. http://dx.doi.org/10.1111/1755-0998.12703

Additionally, please cite the Dryad data package:

Corse E, Meglécz E, Archambaud G, Ardisson M, Martin J, Tougard C, Chappaz R, Dubut V (2017) Data from: A from-benchtop-to-desktop workflow for validating HTS data and for taxonomic identification in diet metabarcoding studies. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.f40v5
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