Data from: Transcriptome analysis reveals novel patterning and pigmentation genes underlying Heliconius butterfly wing pattern variation

Hines HM, Papa R, Ruiz M, Papanicolaou A, Wang C, Nijhout HF, McMillan WO, Reed RD

Date Published: February 5, 2013

DOI: http://dx.doi.org/10.5061/dryad.f76f3

 

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Title Hinesetal_BMCGenomics_fulldatainfo
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Description This file includes accessory information on each transcript contig used from the associated microarray study, including annotation results from various analyses, mean values across probes for that gene across the tested conditions, and significance across the variety of statistical analyses performed. By column this dataset includes: | Transcript ID – The identifications assigned to each transcript on the array. These IDs are referenced in the text and sequences for each contig are available at <http://insectacentral.org>. | Blast2Go Annotation – Annotation term assigned during the Blast2Go analysis of these transcripts. | Flybase Gene ID and Flybase Annotation Abbr. – The gene ID the contig blasted to in Flybase and the name assigned to that gene. | Manual Anno Flybase Gene ID and Manual Anno Flybase Anno Abbr. – Flybase gene identifications and terms for genes that did not hit to Flybase with the contig sequence but were whose identity were determined manually using various parameters outlined in the manuscript. | Significant for Hindwing, Significant for Forewing – If an ID is listed, that gene was significant in either the hindwing or forewing analyses. | Lsmean columns – These provide the mean expression across probes across each array condition, including in the title Race, Stage, and (for forewings) Wing Section. | -log10(p-value) columns– These provide the p-values from ANOVAs for the two listed pairwise comparisons of those Lsmeans | Significant Index columns – Indicate whether p-values of various pairwise comparisons were significant (1) or not (0). | Columns FR – GT- IDs are listed where significant differences were found with various analyses, described in the manuscript. | Columns GU – HM – These refer to the genes that are differentially expressed in an ANOVA along the proximal-distal forewing axis and the significance columns refer to whether there was significance for each of the wing section comparisons. F1 is the basal section, F2 is the medial section, and F3 is the apical section.
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When using this data, please cite the original publication:

Hines HM, Papa R, Ruiz M, Papanicolaou A, Wang C, Nijhout HF, McMillan WO, Reed RD (2012) Transcriptome analysis reveals novel patterning and pigmentation genes underlying Heliconius butterfly wing pattern variation. BMC Genomics 13: 288. http://dx.doi.org/10.1186/1471-2164-13-288

Additionally, please cite the Dryad data package:

Hines HM, Papa R, Ruiz M, Papanicolaou A, Wang C, Nijhout HF, McMillan WO, Reed RD (2012) Data from: Transcriptome analysis reveals novel patterning and pigmentation genes underlying Heliconius butterfly wing pattern variation. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.f76f3
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