Data from: Ligation of glycophorin A generates reactive oxygen species leading to decreased red blood cell function
Khoory, Joseph, Beth Israel Deaconess Medical Center
Estanislau, Jessica, Beth Israel Deaconess Medical Center
Elkhal, Abdallah
Lazaar, Asmae, Beth Israel Deaconess Medical Center
Melhorn, Mark I., Beth Israel Deaconess Medical Center
Brodsky, Abigail, Beth Israel Deaconess Medical Center
Illigens, Ben, Beth Israel Deaconess Medical Center
Hamachi, Itaru, Kyoto University
Kurishita, Yasutaka, Kyoto University
Ivanov, Alexander R., Northeastern University
Shevkoplyas, Sergey, University of Houston
Shapiro, Nathan I., Beth Israel Deaconess Medical Center, Harvard Medical School
Ghiran, Ionita C., Beth Israel Deaconess Medical Center
Published Jan 21, 2016 on Dryad.
https://doi.org/10.5061/dryad.f862c
Cite this dataset
Khoory, Joseph et al. (2016). Data from: Ligation of glycophorin A generates reactive oxygen species leading to decreased red blood cell function [Dataset]. Dryad. https://doi.org/10.5061/dryad.f862c
Abstract
Acute, inflammatory conditions associated with dysregulated complement activation are characterized by significant increases in blood concentration of reactive oxygen species (ROS) and ATP. The mechanisms by which these molecules arise are not fully understood. In this study, using luminometric- and fluorescence-based methods, we show that ligation of glycophorin A (GPA) on human red blood cells (RBCs) results in a 2.1-fold, NADPH-oxidase-dependent increase in intracellular ROS that, in turn, trigger multiple downstream cascades leading to caspase-3 activation, ATP release, and increased band 3 phosphorylation. Functionally, using 2D microchannels to assess membrane deformability, GPS-ligated RBCs travel 33% slower than control RBCs, and lipid mobility was hindered by 10% using fluorescence recovery after photobleaching (FRAP). These outcomes were preventable by pretreating RBCs with cell-permeable ROS scavenger glutathione monoethyl ester (GSH-ME). Our results obtained in vitro using anti-GPA antibodies were validated using complement-altered RBCs isolated from control and septic patients. Our results suggest that during inflammatory conditions, circulating RBCs significantly contribute to capillary flow dysfunctions, and constitute an important but overlooked source of intravascular ROS and ATP, both critical mediators responsible for endothelial cell activation, microcirculation impairment, platelet activation, as well as long-term dysregulated adaptive and innate immune responses.
Usage notes
Flow Cytometry Data of Sepsis Study
Flow cytometry data including C3d, C4d, glycocalyx (WGA), and some other data (FRAP, etc.)
Flow Cytometry Data.xlsx
M606 E0 + WGA
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
M606 E0
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
M606 E24 + WGA
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
M606 E24
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
M613E24-control C3d
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
control C3d.011
M613E24-control C4d
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
control C4d.009
M613E24-control DAF
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
control DAF.005
M613E24-control IgG
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
control IgG.007
M613E24-control RBC + WGA
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
control RBC + WGA.002
M613E24-control RBC
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
control RBC.001
M613E24-sepsis C3d
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
sepsis C3d.012
M613E24-sepsis C4d
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
sepsis C4d.010
M613E24-sepsis DAF
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
sepsis DAF.006
M613E24-sepsis IgG
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
sepsis IgG.008
M613E24-sepsis RBC + WGA
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
sepsis RBC + WGA.004
M613E24-sepsis RBC
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
sepsis RBC.003
M613E48-control C3d
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
control C3d.013
M613E48-control C4d
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
control C4d.012
M613E48-control Ca 2+
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
control Ca 2+.006
M613E48-control IgG
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
control IgG.009
M613E48-control NO
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
control NO.007
M613E48-control RBC + WGA
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
control RBC + WGA.003
M613E48-control RBC
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
control RBC.001
M613E48-sepsis C3d
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
sepsis C3d.014
M613E48-sepsis C4d
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
sepsis C4d.011
M613E48-sepsis Ca 2+
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
sepsis Ca 2+.005
M613E48-sepsis IgG
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
sepsis IgG.010
M613E48-sepsis NO
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
sepsis NO.008
M613E48-sepsis RBC + WGA
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
sepsis RBC + WGA.004
M613E48-sepsis RBC
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
sepsis RBC.002
M616E0-normal RBC + c3d
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
normal RBC + c3d.004
M616E0-normal RBC + c4d
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
normal RBC + c4d.005
M616E0-normal RBC + IgG
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
normal RBC + IgG.003
M616E0-normal RBC + WGA
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
normal RBC + WGA.002
M616E0-Untreated normal RBC
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Untreated normal RBC.001
M616E0 RBC + c3d
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
sepsis M616E0 RBC + c3d.009
M616E0 RBC + c4d
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
sepsis M616E0 RBC + c4d.010
M616E0 RBC + IgG
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
sepsis M616E0 RBC + IgG.008
M616E0 RBC + WGA
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
sepsis M616E0 RBC + WGA.007
M616E0 RBC
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
sepsis M616E0 RBC.006
M616E24-Control RBC +IgG
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Control RBC +IgG.005
M616E24-Control RBC+C3d
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Control RBC+C3d.007
M616E24-Control RBC+C4d
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Control RBC+C4d.009
M616E24-Control RBC+DAF
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Control RBC+DAF.011
M616E24 RBC+C4d
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
M616E24 RBC+DAF
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
M616E24 RBC
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
M616E24.003
M616E24+WGA
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
M616E24RBC +IgG
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
M616E24RBC+C3d
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
M616E24-Normal Control RBC
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Normal Control RBC.001
M616E24-Normal Control RBC+WGA
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Normal Control RBC+WGA.002
M618E0-Control C3D
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Control C3D.006
M618E0-Control C4D
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Control C4D.007
M618E0-Control DAF
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Control DAF.008
M618E0-Control IgG
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Control IgG.005
M618E0-Control RBC
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Control RBC.001
M618E0-Control RBC+WGA
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Control RBC+WGA.002
M618E0-Sepsis +DAF
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Sepsis +DAF.012
M618E0-Sepsis C3D
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Sepsis C3D.010
M618E0-Sepsis C4D
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Sepsis C4D.011
M618E0-Sepsis IgG
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Sepsis IgG.009
M618E0-Sepsis RBC
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Sepsis RBC.003
M618E0-Sepsis RBC+WGA
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Sepsis RBC+WGA.004
M618E24-Data1
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Data.001
M618E24-Data2
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Data.002
M618E24-Data3
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Data.003
M618E24-Data4
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Data.004
M618E24-Data5
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Data.005
M618E24-Data6
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Data.006
M618E24-Data7
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Data.007
M618E24-Data8
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Data.008
M618E24-Data9
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Data.009
M618E24-Data10
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Data.010
M618E24-Data11
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Data.011
M618E24-Data12
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Data.012
M625E0-Control C3D
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Control C3D.004
M625E0-Control C4D
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Control C4D.005
M625E0-Control DAF
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Control DAF.006
M625E0-Control IgG
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Control IgG.003
M625E0-Control RBC
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Control RBC.001
M625E0-Control RBC+WGA
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Control RBC+WGA.002
M625E0-Sepsis RBC C3D
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Sepsis RBC C3D.010
M625E0-Sepsis RBC C4D
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Sepsis RBC C4D.011
M625E0-Sepsis RBC DAF
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Sepsis RBC DAF.012
M625E0-Sepsis RBC IgG
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Sepsis RBC IgG.009
M625E0-Sepsis RBC E0
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Sepsis RBC E0.007
M625E0-Sepsis RBC E0+WGA
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Sepsis RBC E0+WGA.008
M625E24-control c3d
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
control c3d.011
M625E24-control c4d
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
control c4d.012
M625E24-control IgG
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
control IgG.009
M625E24-control NO
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
control NO.010
M625E24-Control RBC + WGA
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Control RBC + WGA.002
M625E24-Control RBC
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Control RBC.001
M625E24-sepsis c3d
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
sepsis c3d.008
M625E24-sepsis c4d
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
sepsis c4d.006
M625E24-sepsis NO
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
sepsis NO.007
M625E24-sepsis RBC + WGA
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
sepsis RBC + WGA.004
M625E24-sepsis RBC
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
sepsis RBC.003
M625E24-sepsis IgG
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
sepsis IgG.005
M630E24-Control C3D
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Control C3D.004
M630E24-Control C4D
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Control C4D.005
M798E0 Ctrl C3D
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Ctrl C3d
M798E0 Ctrl C4D
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Ctrl C4d
M798E0 Ctrl mIgG
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Ctrl mIgG
M798E0 Ctrl RBC
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Ctrl RBC
M798E0 Ctrl WGA
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Ctrl WGA
M798E0 C3D
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
M798E0 C3d
M798E0 C4D
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
M798E0 C4d
M798E0 mIgG
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
M798E0 RBC
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
M798E0 WGA
Flow cytometry raw data. Flow cytometry files (.fcs) generated using a LSRII flow cytometer.
Location
United States of America