Data from: Estimating and mitigating amplification bias in qualitative and quantitative arthropod metabarcoding

Krehenwinkel H, Wolf M, Lim JY, Rominger AJ, Simison WB, Gillespie RG

Date Published: December 18, 2017

DOI: https://doi.org/10.5061/dryad.fs728

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Title DNA_and_tissue_pools
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Description Taxonomic composition of DNA and tissue pools and recovered read counts for all taxa.
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Title DNAPools.tar
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Description Illumina read files for the different markers and 16 DNA pools. Reads for the mitochondrial and nuclear markers are merged into each one file.
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Title PCRCycles.tar
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Description Illumina read files for the 23 DNA pools of the PCR cycle experiment. The number of PCR cycles for each sample is indicated in the file name.
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Title SingleSpecimens.tar
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Description Illumina read files of all 8 markers sequenced separately for each of the 43 taxa, which were used to prepare the DNA mock communities. Reads for all 8 markers are merged into single files.
Download SingleSpecimens.tar.gz (581.9 Mb)
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Title TissuePools.tar
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Description Illumina read files of the tissue pools. Each pool was sequenced twice, once using degraded DNA (L) and once using intact high molecular weight DNA (H).
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Title gDNA.tar
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Description Illumina read files of the sequenced genomic DNA pools. Due to low coverage, we merged the pools into one file, using equal read numbers from each file.
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When using this data, please cite the original publication:

Krehenwinkel H, Wolf M, Lim JY, Rominger AJ, Simison WB, Gillespie RG (2017) Estimating and mitigating amplification bias in qualitative and quantitative arthropod metabarcoding. Scientific Reports 7(1): 17668. https://doi.org/10.1038/s41598-017-17333-x

Additionally, please cite the Dryad data package:

Krehenwinkel H, Wolf M, Lim JY, Rominger AJ, Simison WB, Gillespie RG (2017) Data from: Estimating and mitigating amplification bias in qualitative and quantitative arthropod metabarcoding. Dryad Digital Repository. https://doi.org/10.5061/dryad.fs728
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