Data from: Effect of marker choice and thermal cycling protocol on zooplankton DNA metabarcoding studies

Clarke LJ, Beard JM, Swadling KM, Deagle BE

Date Published: January 13, 2017

DOI: http://dx.doi.org/10.5061/dryad.gf246

 

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Title 46vsTD_fastq
Downloaded 4 times
Description Raw FASTQ files for COI marker PCR-amplified using either the published touchdown (TD) or a single annealing temperature (46) thermal cycling protocol.
Download 46vsTD_fastq.zip (155.1 Mb)
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Title Cop16S_fastq
Downloaded 4 times
Description Raw FASTQ files for Storm Bay zooplankton assemblages amplified with the Cop16S marker.
Download Cop16S_fastq.zip (46.33 Mb)
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Title Uni18S_fastq
Downloaded 6 times
Description Raw FASTQ files for Storm Bay zooplankton assemblages amplified with the Uni18S marker.
Download Uni18S_fastq.zip (41.49 Mb)
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Title LerayCOI_fastq
Downloaded 5 times
Description Raw FASTQ files for Storm Bay zooplankton assemblages amplified with the Uni18S marker.
Download LerayCOI_fastq.zip (25.77 Mb)
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Title OTU_tables
Downloaded 3 times
Description OTU tables for Storm Bay zooplankton assemblages amplified using three metabarcoding markers (mitochondrial COI, 16S, and nuclear 18S), and the comparison of the COI marker using two different thermal cycling protocols.
Download OTU_tables.zip (8.576 Kb)
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When using this data, please cite the original publication:

Clarke LJ, Beard JM, Swadling KM, Deagle BE (2017) Effect of marker choice and thermal cycling protocol on zooplankton DNA metabarcoding studies. Ecology and Evolution 7(3): 873-883. http://dx.doi.org/10.1002/ece3.2667

Additionally, please cite the Dryad data package:

Clarke LJ, Beard JM, Swadling KM, Deagle BE (2017) Data from: Effect of marker choice and thermal cycling protocol on zooplankton DNA metabarcoding studies. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.gf246
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