Data from: Are Niemann-Pick type C proteins key players in cnidarian-dinoflagellate endosymbioses?

Dani V, Ganot P, Priouzeau F, Furla P, Sabourault C

Date Published: July 29, 2014

DOI: http://dx.doi.org/10.5061/dryad.gs7s3

 

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Title Alignment file of NPC2 protein sequences_Figure1A
Description Sequences of NPC2 proteins aligned using the MAFFT program. This alignment was used to construct a phylogenetic tree using the maximum likelihood method (phyML3.0 software) and the WAG+I+G substitution model (I=0.057 and gamma factor=5.831). Accession number of sequences: AvNPC2-d,HG670301; AvNPC2-c, HG670300; NvNPC2-2, XP_001627355; NvNPC2-3, XP_001635502; AdNPC2-2, adi_v1.06781; AdNPC2-3, adi_v1.23434; AdNPC2-4, adi_v1.10342; AvNPC2-b, HG670299; AdNPC2-1, adi_v1.14987 + contig adi_v1.08570; AdNPC2-5, aug_v2a.08569; AdNPC2-6, aug_v2a.08570; AvNPC2-a, HG670298; NvNPC2-1, XP_001622874; HsNPC2, NP_006423.1; DrNPC2-a, NP_001122191.1; DrNPC2-b, NP_775331.1; CiNPC2-1, XP_002127695.1; TaNPC2, XP_002109765.1; HvNPC2-3, XP_002166396.2; Hv NPC2-4, XP_002166374.2; Hv NPC2-2, XP_002164043.1; Hv NPC2-1, XP_002156875.1; AqNPC2, CL5422Contig1 from AQUE_CAP3_100104 assembly from compagen
Download Alignment file of NPC2 protein sequences_...1A.fas (3.61 Kb)
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Title Alignment file of NPC1 protein sequences_Figure2
Description Sequences of NPC1 proteins aligned using the MAFFT program. This alignment was used to generate a phylogenetic tree using the phyML 3.0 sofware, the maximum likelihood method and the LG+I+G substitution model (I=0.1 and gamma factor=1.266). Accession numbers: HsNPC1L1, NP_001095118.1; BtNPC1L1, XP_588051; DrNPC1L1, XP_002663230.2; CiNPC1-b, XP_002122922; DmNPC1-a, NP_609357; DmNPC1-b, NP_608417; HsNPC1, AAK25791.1; BtNPC1, AAI51277; DrNPC1, XP_001919958.3; CiNPC1-a, XP_002120129; SpNPC1, XP_780036; AvNPC1, HG670297; NvNPC1, XM_001634881.1; AdNPC1, adi_v1.19271 from ADIG_G-PEP_111201 database; HmNPC1, XM_002164383.1; AqNPC1, XP_003384854.1;
Download Alignment file of NPC1 protein sequences_...e2.fas (16.12 Kb)
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Title PrimersEfficiency
Description PCR efficiency of primers used in this study (DNA quantification and heat stress experiment)
Download PrimerEfficiency.xlsx (11.64 Kb)
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Title qPCR_QuantifZxAv
Description Symbiodinium quantification by real-time quantitative PCR measurement of the relative nuclear gene copy number (symbiont to host nuclei) during hyperthermal stress.
Download qPCR_QuantifZxAv.xlsx (10.72 Kb)
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Title qPCR_HeatStress
Description Relative gene expression data analysis (raw Ct and normalized expression data) for the heat stress experiment
Download qPCR_HeatStress.xlsx (18.48 Kb)
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Title Normalizationfactor_HeatStress
Description Normalization factor was calculated based on the expression level of RCC2 and COP-ɣ genes using the GeNorm software, for the heat stress experiment.
Download Normalizationfactor_HeatStress.xlsx (10.68 Kb)
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Title TreeFile_NPC2_Figure1
Description Tree data generated using the maximum likelihood method and the PhyML3.0 software. Compute these data with the SeaView program to display the NPC2 phylogenetic tree.
Download TreeFile_NPC2_Figure1.txt (810 bytes)
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Title TreeFile_NPC1_Figure2
Description Tree data generated using the maximum likelihood method and the PhyML3.0 software. Compute these data with the SeaView program to display the NPC1 phylogenetic tree.
Download TreeFile_NPC1_Figure2.txt (556 bytes)
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When using this data, please cite the original publication:

Dani V, Ganot P, Priouzeau F, Furla P, Sabourault C (2014) Are Niemann-Pick type C proteins key players in cnidarian-dinoflagellate endosymbioses? Molecular Ecology 23(18): 4527-4540. http://dx.doi.org/10.1111/mec.12876

Additionally, please cite the Dryad data package:

Dani V, Ganot P, Priouzeau F, Furla P, Sabourault C (2014) Data from: Are Niemann-Pick type C proteins key players in cnidarian-dinoflagellate endosymbioses? Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.gs7s3
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