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dc.contributor.author Vancevska, Aleksandra
dc.contributor.author Douglass, Kyle M.
dc.contributor.author Pfeiffer, Verena
dc.contributor.author Manley, Suliana
dc.contributor.author Lingner, Joachim
dc.date.accessioned 2017-04-18T19:25:54Z
dc.date.available 2017-04-18T19:25:54Z
dc.date.issued 2017-04-05
dc.identifier doi:10.5061/dryad.h1157
dc.identifier.citation Vancevska A, Douglass KM, Pfeiffer V, Manley S, Lingner J (2017) The telomeric DNA damage response occurs in the absence of chromatin decompaction. Genes & Development 31: 567-577.
dc.identifier.uri http://hdl.handle.net/10255/dryad.140536
dc.description Telomeres are specialized nucleoprotein structures that protect chromosome ends from DNA damage response (DDR) and DNA rearrangements. The telomeric shelterin protein TRF2 suppresses the DDR, and this function has been attributed to its abilities to trigger t-loop formation or prevent massive decompaction and loss of density of telomeric chromatin. Here, we applied stochastic optical reconstruction microscopy (STORM) to measure the sizes and shapes of functional human telomeres of different lengths and dysfunctional telomeres that elicit a DDR. Telomeres have an ovoid appearance with considerable plasticity in shape. Examination of many telomeres demonstrated that depletion of TRF2, TRF1, or both affected the sizes of only a small subset of telomeres. Costaining of telomeres with DDR markers further revealed that the majority of DDR signaling telomeres retained a normal size. Thus, DDR signaling at telomeres does not require decompaction. We propose that telomeres are monitored by the DDR machinery in the absence of telomere expansion and that the DDR is triggered by changes at the molecular level in structure and protein composition.
dc.relation.haspart doi:10.5061/dryad.h1157/1
dc.relation.haspart doi:10.5061/dryad.h1157/2
dc.relation.haspart doi:10.5061/dryad.h1157/3
dc.relation.haspart doi:10.5061/dryad.h1157/4
dc.relation.haspart doi:10.5061/dryad.h1157/5
dc.relation.haspart doi:10.5061/dryad.h1157/6
dc.relation.haspart doi:10.5061/dryad.h1157/7
dc.relation.haspart doi:10.5061/dryad.h1157/8
dc.relation.haspart doi:10.5061/dryad.h1157/9
dc.relation.haspart doi:10.5061/dryad.h1157/10
dc.relation.haspart doi:10.5061/dryad.h1157/11
dc.relation.haspart doi:10.5061/dryad.h1157/12
dc.relation.isreferencedby doi:10.1101/gad.294082.116
dc.subject Telomeres
dc.subject STORM
dc.title Data from: The telomeric DNA damage response occurs in the absence of chromatin decompaction
dc.type Article
dc.contributor.correspondingAuthor Lingner, Joachim
prism.publicationName Genes & Development

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