Data from: The genetic architecture of UV floral patterning in sunflower

Moyers BT, Owens GL, Baute GJ, Rieseberg LH

Date Published: May 3, 2017

DOI: http://dx.doi.org/10.5061/dryad.h21v2.2

 

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Title Flowerheads photographed in UV
Downloaded 16 times
Description Zipped directory containing JPG files of Helianthus argophyllus flowerheads photographed with a Nikon D70s digital camera fitted with a Noflexar 35mm lens and a reverse-mounted 2 inch Baader U-Filter (restricting the transmission of light to 320–380 nm). Files are named according to the plant's location in the field and the photograph number (e.g. 'b1-11DSC_1586' is the plant from row 1, space 11, photograph 1586; exceptions are the F1 individuals which are labeled X3A-# or X3B-# instead of by field location). The archived data file with phenotypic measurements contains the key to translate field location to plant genotype.
Download UVphotos.zip (679.5 Mb)
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Title Custom Perl script for formatting paired-end GBS data for uneak pipeline
Downloaded 14 times
Description This script translates paired-end fastq sequencing files into two independent sets of individually barcoded reads with a custom Perl script, to be used in the uneak pipeline (which is built only for single-end reads, from TASSEL 3; Lu et al., 2013). usage: perl GBS_Fastq_BarcodeAdder.pl uneak_key_file.txt library_1.fq library_2.fq combined_library_out.fq
Download GBS_Fastq_BarcodeAdder_2Enzyme.pl (2.071 Kb)
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Title Bash commands used to filter for final SNP matrix
Downloaded 9 times
Description This file contains the series of bash commands used to filter uneak output to call genotypes using more stringent filters. Starting with the HapMapCount file (for each individual at each tag pair: minimum of 10 tags, heterozygote if the ratio of the two tags < 10, homozygote if the ratio of the two tags > 50, otherwise N), filter the resulting file for sites with alternate homozygous genotypes in the two parent individuals (7,875 sites), filter those sites for a minimum coverage of 50% across all individuals and observed heterozygousity of 0.3–0.7, and then format these genotypes for import into R/qtl.
Download uneak_bash_filters.txt (3.233 Kb)
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Title Phenotype and genotype matrix for QTL mapping
Downloaded 15 times
Description Data matrix formatted for R/qtl, for QTL mapping in F2 population of Helianthus argophyllus. Column headers are: GBS_name (sample identifier in sequence files), name (field location, e.g. R1-11 is row 1, space 11), id (numeric identifier unique to individual), fam (F2 family), row (row location), , place (place within row location), mom (identity of F1 maternal parent), uvR (proportional UV pigmentation), uvD (absolute UV bullseye diameter, cm), total.d.cm (total flowerhead diameter, cm), disc.d (disc diameter, cm), petal.d.cm (mean ligule length, cm), FHage (binned maturity score of flowerhead). The following columns are marker genotypes, with the map location of those markers on rows 2 (linkage group) and 3 (centiMorgan).
Download map3all_dryad_phenoUV_FHage.csv (546.8 Kb)
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Title Phenotypic data for all individuals
Downloaded 10 times
Description Includes phenotypes for all USDA accession, F1, and F2 individuals measured in this study. Column headers are: Dataset (Main = F2, others as indicated), name (plant location in field, e.g. R1-11 is row 1, space 11), filename (file name of flowerhead photograph), FHage (binned maturity of sampled flowerhead), clip (length in pixels of standard object in photo), pixels_cm (number of pixels per cm for this photo), clip_size (which standard object was included in photo), class (population to which individual belonged, e.g. F2), cytoplasm (whether F2 individual had ARG-1805 as maternal grandmother, A, or ARG-1834, B), F1pop (F1 family for F2 individuals), session (photography session), picN (photo number), h.disc (width of flowerhead disc in pixels), v.disc (height of flowerhead disc in pixels), 1f (total length of first measured ligule in pixels),1p (UV pigmented length of first measured ligule in pixels), 2f (total length of second measured ligule in pixels), 2p (UV pigmented length of second measured ligule in pixels), 3f (total length of third measured ligule in pixels), 3p (UV pigmented length of second measured ligule in pixels), r1 (1p/1f) , r2 (2p/2f), r3 (3p/3f), mean.r (mean of r1, r2, r3, or proportional UV pigmentation), uv.d (mean of h.disc and v.disc plus 2 x mean of 1p, 2p, 3p), uv.d.cm (uv.d divided by pixels_cm, or absolute UV bullseye diameter in cm), total.d.cm (total flowerhead diameter in cm), disc.d (mean of h.disc and v.disc divided by pixels_cm, or disc diameter in cm), petal.d.cm (mean of 1f, 2f, 3f divided by pixels_cm, or ligule length in cm).
Download UVdata_class_dryad.csv (110.6 Kb)
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Title Candidate genes for UV floral patterning
Downloaded 7 times
Description List of candidate genes under the four proportional UV pigmentation QTL matched against the Arabidopsis thaliana TAIR10 protein database using blastp (e < e-20; Lamesch et al., 2011). Filtered specifically for proteins with WD or bHLH functional domains or part of the MYB or UDP-glycotransferase families. Column headers: which (filtered functional category), HaName (id of Helianthus annuus mRNA), AtName (best Arabidopsis thaliana hit), evalue (match value), chr (Helianthus annuus chromosome number), program (maker for this analysis), type (all genes in this analysis), start (start of gene in basepairs), end (end of gene in basepairs), score (NA), strand (+ or -), phase (NA), ID (internal id), Name (internal name), followed by functional annotation of that protein.
Download all4_UVqtl_blastsUniq.txt (103.8 Kb)
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Title All genes under proportional UV pigmentation QTL
Downloaded 6 times
Description List of all genes under the proportional UV pigmentation QTL. Column headers: chr (chromosome in Helianthus annuus genome), start (first basepair location of gene on chromosome), end (last basepair location of gene on chromosome), and HaName (gene name). More information about these genes is available at https://sunflowergenome.org/
Download all_QTL_genes.txt (321.8 Kb)
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When using this data, please cite the original publication:

Moyers BT, Owens GL, Baute GJ, Rieseberg LH (2017) The genetic architecture of UV floral patterning in sunflower. Annals of Botany 120(1): 39-50. http://dx.doi.org/10.1093/aob/mcx038

Additionally, please cite the Dryad data package:

Moyers BT, Owens GL, Baute GJ, Rieseberg LH (2017) Data from: The genetic architecture of UV floral patterning in sunflower. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.h21v2.2
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