Show simple item record Balakirev, Evgeniy S. Krupnova, Tatiana N. Ayala, Francisco J.
dc.coverage.spatial the Sea of Japan 2012-11-12T18:38:44Z 2012-11-12T18:38:44Z 2012-07-11
dc.identifier doi:10.5061/dryad.h32m962j
dc.identifier.citation Balakirev ES, Krupnova TN, Ayala FJ (2012) DNA variation in the phenotypically-diverse brown alga Saccharina japonica. BMC Plant Biology, 12:108.
dc.description BACKGROUND: Saccharina japonica (Areschoug) Lane, Mayes, Druehl et Saunders is an economically important and highly morphologically variable brown alga inhabiting the northwest Pacific region. On the basis of nuclear (ITS), plastid (rbcLS) and mitochondrial (COI) DNA sequence data, we have analyzed the genetic composition of typical Saccharina japonica (TYP) and its two common morphological varieties, known as the “longipes” (LON) and “shallow-water” (SHA) forms seeking to clarify their taxonomical status and to evaluate the possibility of cryptic species within S. japonica. RESULTS: The data show that the TYP and LON forms are very similar genetically in spite of drastic differences in morphology, life history traits, and ecological preferences. Both, however, are genetically quite different from the SHA form. The two Saccharina lineages are distinguished by 109 fixed single nucleotide differences as well as by seven fixed length polymorphisms (based on a 4,286 bp concatenated dataset that includes three gene regions). The GenBank database reveals a close affinity of the TYP and LON forms to S. japonica and the SHA form to S. cichorioides. The three gene markers used in the present work have different sensitivity for the algae species identification. The COI gene is the most discriminant gene marker. However, the 5’-COI “barcoding” region (~ 650 bp) is not satisfactory and even misleading for identification purposes, at least in the case of algae species that might have experienced historical hybridization events. CONCLUSION: Taking into account the potential roles of phenotypic plasticity in evolution, we conclude that the TYP and LON forms represent examples of algae phenotypic diversification that enables successful adaptation to contrasting shallow- and deep-water marine environments, while the SHA form is very similar to S. cichorioides and should be considered a different species. Practical applications for algal management and conservation are shortly considered.
dc.relation.haspart doi:10.5061/dryad.h32m962j/1
dc.relation.haspart doi:10.5061/dryad.h32m962j/2
dc.relation.isreferencedby doi:10.1186/1471-2229-12-108
dc.relation.isreferencedby PMID:22784095
dc.subject brown algae
dc.subject kelp
dc.subject Saccharina
dc.subject DNA polymorphism
dc.subject phenotypic plasticity
dc.subject Saccharina japonica morphological forms
dc.title Data from: DNA variation in the phenotypically-diverse brown alga Saccharina japonica
dc.type Article *
dwc.ScientificName Saccharina japonica
dwc.ScientificName Saccharina cichorioides
dc.contributor.correspondingAuthor Balakirev, Evgeniy S.
prism.publicationName BMC Plant Biology
dryad.dansTransferDate 2017-09-26T13:18:15.300-0400
dryad.dansTransferDate 2017-10-31T14:23:20.592-0400

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Description The data represent the alignment of rbcLS, COI, and ITS in 29 algae samples.
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Title Concatenate_NJ_tree_Saccharina_rbcLS_COI_ITS
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Description The tree shows relationships between morphological forms of Saccharina japonica.
Download Concatenate_NJ_tree_Saccharina_rbcLS_COI_ITS.ppt (101.8 Kb)
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