Data from: The adaptive potential of subtropical rainbowfish in the face of climate change: heritability and heritable plasticity for the expression of candidate genes

McCairns SRJ, Smith S, Sasaki M, Bernatchez L, Beheregaray LB

Date Published: January 19, 2016

DOI: http://dx.doi.org/10.5061/dryad.h40d8

 

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Title mRNA transcription of candidate genes for thermal adaptation in the crimson-spotted rainbowfish.
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Description Total mRNA was extracted from laboratory bred and reared individuals (F1); parents were wild-caught and originated from the Brisbane River. At 6 months of age, individuals from each family group were subjected to one of two thermal treatments for a duration of 80 days. Rearing tank (Tank), sex (Sex), thermal treatment group (Treat) individual identifier (animal), parents (DAM & SIRE) and full-sib family group (Fam) are provided for each individual. Immediately following thermal treatment, fish were euthanized and total mRNA extracted from liver tissues. Transcript abundance was estimated via quantitative real-time PCR. LinRegPCR was used to convert raw CT values into estimates of initial/starting mRNA concentrations (N0). Initial mRNA concentrations were standardized relative to the geometric mean of two reference genes (geomean.REFS). Standardized values of candidate genes occupy the final 12 columns; gene symbols (column headings) are the same as those used in the accompanying article (McCairns et al. 2016).
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When using this data, please cite the original publication:

McCairns S, Smith S, Sasaki M, Bernatchez L, Beheregaray L (2016) The adaptive potential of subtropical rainbowfish in the face of climate change: heritability and heritable plasticity for the expression of candidate genes. Evolutionary Applications 9(4): 531–545. http://dx.doi.org/10.1111/eva.12363

Additionally, please cite the Dryad data package:

McCairns SRJ, Smith S, Sasaki M, Bernatchez L, Beheregaray LB (2016) Data from: The adaptive potential of subtropical rainbowfish in the face of climate change: heritability and heritable plasticity for the expression of candidate genes. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.h40d8
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