Data from: Pleiotropy and the low cost of individual traits promote cooperation

Mitri S, Foster KR

Date Published: January 5, 2016

DOI: http://dx.doi.org/10.5061/dryad.h5fg1

 

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Title CFUs of mixes of strains in CAA and BSA at 0 and 48 hours
Downloaded 11 times
Description Population sizes of each of the strains (wildtype WT, lasB mutants DLB and lasR mutants DLR) were quantified in monoculture and in coculture at different ratios of WT to mutant (9:1, 1:1 or 1:9). Experiments were conducted in two growth media: CAA (casamino acids + M9), which all strains could take up and BSA (casamino acids + M9 + BSA, which could only be digested using elastase). To distinguish the strains in cocultures, fluorescently-labelled strains (YFP or DsRed) were used and mixed in all possible combinations to test for labelling effects. We quantified population sizes by plating out dilution series and counting colony-forming units. These are listed in the file for t=0h and t=48h. This shows how the ratio of the strains and the total population size change throughout the experiment. These data were used to generate Fig. 1B, 2B and 3.
Download CFUs_CAA_BSA_0h_48h.xlsx (58.30 Kb)
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Title Growth curves (OD_600) over 48 hours in CAA medium
Downloaded 9 times
Description Population sizes of each of all experimental treatments (monocultures of wildtype WT, lasB mutants DLB and lasR mutants DLR) and cocultures of WT and one of the mutants at different ratios (9:1, 1:1 or 1:9) growing in CAA medium (casamino acids, which all strains should be able to take up). We quantified population sizes using a Tecan plate reader measuring OD_600. These are listed in the file every hour for 48 hours. These data were used to generate Fig. 1A.
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Title Growth curves (OD_600) over 48 hours in BSA medium
Downloaded 7 times
Description Population sizes of each of all experimental treatments (monocultures of wildtype WT, lasB mutants DLB and lasR mutants DLR) and cocultures of WT and one of the mutants at different ratios (9:1, 1:1 or 1:9) growing in BSA medium (casamino acids with BSA, which can only be degraded by elastase). We quantified population sizes using a Tecan plate reader measuring OD_600. These are listed in the file every hour for 48 hours. These data were used to generate Fig. 2A.
Download growth_curves_BSA.xlsx (198.8 Kb)
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When using this data, please cite the original publication:

Mitri S, Foster KR (2016) Pleiotropy and the low cost of individual traits promote cooperation. Evolution 70(2): 488–494. http://dx.doi.org/10.1111/evo.12851

Additionally, please cite the Dryad data package:

Mitri S, Foster KR (2016) Data from: Pleiotropy and the low cost of individual traits promote cooperation. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.h5fg1
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