Data from: An extracellular biochemical screen reveals that FLRTs and Unc5s mediate neuronal subtype recognition in the retina

Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray Thomas A., Kay JN, Wojtowicz WM

Date Published: April 8, 2016

DOI: http://dx.doi.org/10.5061/dryad.hf50r

 

Files in this package

Content in the Dryad Digital Repository is offered "as is." By downloading files, you agree to the Dryad Terms of Service. To the extent possible under law, the authors have waived all copyright and related or neighboring rights to this data. CC0 (opens a new window) Open Data (opens a new window)

Title Mouse retina extracellular receptor-ligand biochemical screen
Downloaded 19 times
Description Biochemical screen for extracellular receptor-ligand interactions in the developing mouse retina. The extracellular domain (ECD) region of 126 cell surface or secreted proteins were tested for pairwise binding in a matrix. ECDs along the x-axis were C-terminally fused to the Fc region of human IgG1. ECDs along the y-axis were C-terminally fused to alkaline phosphatase (AP). Proteins were tested for binding using a high-throughput, ELISA-based binding assay. In this assay, ECD-AP receptor protein is captured on an ELISA plate using an anti-AP antibody. Binding of ECD-Fc ligand protein to the ECD-AP receptor protein is detected by inclusion of an anti-Fc antibody conjugated to HRP. HRP activity is measured using O.D. 650 nm one hour following addition of substrate. Raw data values are reported.
Download Visser et al Biochemical Screen Data for D....xlsx (106.1 Kb)
Details View File Details

When using this data, please cite the original publication:

Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray TA, Kay JN, Wojtowicz WM (2015) An extracellular biochemical screen reveals that FLRTs and Unc5s mediate neuronal subtype recognition in the retina. eLife 4: e08149. http://dx.doi.org/10.7554/eLife.08149

Additionally, please cite the Dryad data package:

Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray Thomas A., Kay JN, Wojtowicz WM (2015) Data from: An extracellular biochemical screen reveals that FLRTs and Unc5s mediate neuronal subtype recognition in the retina. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.hf50r
Cite | Share
Download the data package citation in the following formats:
   RIS (compatible with EndNote, Reference Manager, ProCite, RefWorks)
   BibTex (compatible with BibDesk, LaTeX)

Search for data

Be part of Dryad

We encourage organizations to: