Data from: A species-specific multigene family mediates differential sperm displacement in Drosophila melanogaster

Jayawal V, Jimenez J, Magie R, Nguyen K, Clifton B, Yeh S, Ranz JM

Date Published: December 13, 2017

DOI: https://doi.org/10.5061/dryad.j1t17

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Title Sperm counts in the reproductive tract of doubly-mated females
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Description Assays were performed to evaluate how sperm dynamics in the reproductive tract of doubly-mated females differs when the second-to-mate experimental male is either possessing (Sdic+ ) or lacking (Sdic- ) the entire D. melanogaster-specific Sdic multigene family. Virgin females were first mated to a reference Sdic+ male and then to one of the two possible experimental males (Sdic+ or Sdic- ) two days later. Females were randomly assigned to one of three time points after the end of the second mating period (30 min, 1 h and 45 min, and 2 days) and subsequently flash frozen. Female reproductive tracts were dissected and the sperm from the different males were counted using a fluorescent microscope. Reference and experimental sperm fluoresce red and green, respectively.Sperm counting was performed for the bursa and both sperm storage organs, i.e. the paired spermatheca and the seminal receptacle.
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When using this data, please cite the original publication:

Jayaswal V, Jimenez J, Magie R, Nguyen K, Clifton B, Yeh S, Ranz JM (2018) A species-specific multigene family mediates differential sperm displacement in Drosophila melanogaster. Evolution, online in advance of print. https://doi.org/10.1111/evo.13417

Additionally, please cite the Dryad data package:

Jayawal V, Jimenez J, Magie R, Nguyen K, Clifton B, Yeh S, Ranz JM (2018) Data from: A species-specific multigene family mediates differential sperm displacement in Drosophila melanogaster. Dryad Digital Repository. https://doi.org/10.5061/dryad.j1t17
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