Data from: Differential gene expression according to race and host plant in the pea aphid

Eyres I, Jaquiéry J, Sugio A, Duvaux L, Gharbi K, Zhou J, Legeai F, Nelson M, Simon J, Smadja CM, Butlin R, Ferrari J

Date Published: July 28, 2016

DOI: http://dx.doi.org/10.5061/dryad.j449c

 

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Title raw_count
Downloaded 9 times
Description Script used to calculate the number of reads mapped to each gene from the Official Gene Set annotation from AphidBase (Legeai et al. 2010), by summing each read that overlaps at least one exon of any particular gene.
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Title A. pisum raw counts
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Description Transcriptome data from 52 aphid genotypes, reared both on Vicia faba and on their preferred host plant. Illumina sequencing performed using HiSeq2000 using 75 bp paired-end reads (v3 chemistry). Reads mapped to the pea aphid reference genome (IAGC) for each sample separately using TopHat2 (v2.08). The number of reads mapped to each gene from the Official Gene Set annotation from AphidBase (http://aphidbase.com/) was calculated by summing each read that overlaps at least one exon of any particular gene. Read counts reported for 36,401 genes.
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When using this data, please cite the original publication:

Eyres I, Jaquiéry J, Sugio A, Duvaux L, Gharbi K, Zhou J, Legeai F, Nelson M, Simon J, Smadja CM, Butlin R, Ferrari J (2016) Differential gene expression according to race and host plant in the pea aphid. Molecular Ecology 25(17): 4197-4215. http://dx.doi.org/10.1111/mec.13771

Additionally, please cite the Dryad data package:

Eyres I, Jaquiéry J, Sugio A, Duvaux L, Gharbi K, Zhou J, Legeai F, Nelson M, Simon J, Smadja CM, Butlin R, Ferrari J (2016) Data from: Differential gene expression according to race and host plant in the pea aphid. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.j449c
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