Data from: NF-κB oscillations translate into functionally related patterns of gene expression

Zambrano S, De Toma I, Piffer A, Bianchi ME, Agresti A

Date Published: February 11, 2016

DOI: http://dx.doi.org/10.5061/dryad.j62n1

 

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Title Microarray data; chronic and pulsed stimulations of TNFalpha
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Description GFP-p65 MEFs were either chronically or periodically stimulated (90' and 180' forcing periods) with TNF-alpha. RNA samples were extracted at selected timepoints using the Illustra RNAspin Mini kit (GE Healthcare). Following extraction, RIN (RNA Integrity Number) was >9 (BioAnalyser, Agilent RNA Nano Kit). RNA samples (500 ng) were reverse transcribed with the Illumina TotalPrep RNA Amplification Kit (Ambion) and copy RNA (cRNA) was generated with 14 hour in vitro transcription reactions and checked at the BioAnalyser. Washing, staining, and hybridization were performed according to standard Illumina protocols. cRNA samples were then hybridized to Illumina BeadChip Array MouseRefSeq-8 v2. BeadChips were scanned with BeadArray™ Reader in channel 2. The .zip file contains the following files: Quality control files: "Zambrano_90MIN_ControlProbe.csv", "Zambrano_180MIN_ControlProbe.csv", "Zambrano_Chronic_ControlProbe.csv". Raw data files: "ZambranoMicroarray_90MIN.csv", "ZambranoMicroarray_180MIN.csv", "ZambranoMicroarray_Chronic.csv".
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When using this data, please cite the original publication:

Zambrano S, De Toma I, Piffer A, Bianchi ME, Agresti A (2016) NF-κB oscillations translate into functionally related patterns of gene expression. eLife 5: e09100. http://dx.doi.org/10.7554/eLife.09100

Additionally, please cite the Dryad data package:

Zambrano S, De Toma I, Piffer A, Bianchi ME, Agresti A (2016) Data from: NF-κB oscillations translate into functionally related patterns of gene expression. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.j62n1
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