Data from: Pharmacological inhibition of cystine-glutamate exchange induces endoplasmic reticulum stress and ferroptosis

Dixon SJ, Patel DN, Welsch M, Skouta R, Lee ED, Thomas AG, Gleason CE, Tatonetti N, Slusher BS, Stockwell BR

Date Published: May 22, 2014

DOI: http://dx.doi.org/10.5061/dryad.jp43c

 

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Title Data Package 1
Description FPKM expression values from RNA-seq using HT-1080 fibrosarcoma cells under four different conditions, with samples submitted in two biological replicates. The column titles map to these four conditions: 1) DMSO (no treatment), 2) B-ME (ferroptosis inhibitor), 3) Erastin (ferroptosis inducer), and 4) Erastin + B-ME. Fold changes were calculated for Erastin/DMSO and Erastin+B-ME/DMSO. For these experiments, only genes with FPKM not equal to zero in any condition and FPKM≥1 for both replicates in the DMSO condition were considered in the analysis. To further restrict the analysis to high quality data, we only examined those genes where the difference between replicate values in the two DMSO and the two erastin-treated samples was <2.5x.
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Title Data Package 2
Description FPKM expression values from RNA-seq using parental DU-145 prostate cancer cells and clonal cell lines derived from the parental line with resistance to erastin-induced ferroptosis. Sample nomenclature is as follows: "2.5E-2" means this resistant line was produced using a selective pressure of 2.5uM Erastin, and was the second clone evaluated at that concentration. The column titles map to one parental and five resistant clonal lines tested: DU-145 (parental, sensitive), 2.5E-1, 2.5E-2, 2.5E-3, 2.5E-5, and 2.6E-23 (all resistant). Fold changes were calculated using the ratio of each resistant line/parental (column directly to the right of each resistant line). Average fold changes (resistant/parental, column M) and average ΔFPKM (resistant - parental, column N) for each gene across all resistant lines were calculated. For these experiments, only genes with FPKM > 0.1 in the DU-145 condition and FPKM ≥ 1 for all other conditions were evaluated.
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Title Extended Materials & Methods
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When using this data, please cite the original publication:

Dixon SJ, Patel DN, Welsch M, Skouta R, Lee ED, Hayano M, Thomas AG, Gleason CE, Tatonetti N, Slusher BS, Stockwell BR (2014) Pharmacological inhibition of cystine-glutamate exchange induces endoplasmic reticulum stress and ferroptosis. eLife 3: e02523. http://dx.doi.org/10.7554/eLife.02523

Additionally, please cite the Dryad data package:

Dixon SJ, Patel DN, Welsch M, Skouta R, Lee ED, Thomas AG, Gleason CE, Tatonetti N, Slusher BS, Stockwell BR (2014) Data from: Pharmacological inhibition of cystine-glutamate exchange induces endoplasmic reticulum stress and ferroptosis. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.jp43c
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