Data from: Genetic barcoding of dark-spored myxomycetes (Amoebozoa)—Identification, evaluation and application of a sequence similarity threshold for species differentiation in NGS studies

Borg Dahl M, Brjenrod AD, Unterseher M, Hoppe T, Feng Y, Novozhilov Y, Sørensen SJ, Schnittler M

Date Published: October 10, 2017

DOI: https://doi.org/10.5061/dryad.jv77v

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Title 502-705_S22_L001_R2_001
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Description Raw data, forward reads
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Title 502-705_S22_L001_R2_001
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Description Raw data, reverse reads
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Title 502705map
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Description A mapping file to describe the barcode combinations for demultiplexing.
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Title cau demultiplexing script
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Description Demultiplexing script for the caucasus data. Requires uparse 7 and biopieces installed.
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Title post-processing of caucasus data after demultiplexing
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Description post-processing of caucasus data after demultiplexing. Requires uparse 7 including support scripts and NCBI blast
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Title cau_demultiplexed
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Description Demultiplexed fastq files produced by cau_fastq.py
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Title cau_demultiplexed_trimmed
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Description Demultiplexed fastq files with barcodes removed.
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Title cau_fastq
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Description Script for preprocessing raw miseq output data.
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Title S6_Scripts_Resubmission
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Description Supplementary S6 from the manuscript. Contains scripts and commandlines necessary for reproducing the conclusions of the manuscript
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Title README
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Description README file for the data
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Title Database_MAFFT_incl_outlier_20170725
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Description The database of Myxomycete sequences described in the paper.
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When using this data, please cite the original publication:

Borg Dahl M, Brejnrod AD, Unterseher M, Hoppe T, Feng Y, Novozhilov Y, Sørensen SJ, Schnittler M (2017) Genetic barcoding of dark-spored myxomycetes (Amoebozoa)-Identification, evaluation and application of a sequence similarity threshold for species differentiation in NGS studies. Molecular Ecology Resources, online in advance of print. https://doi.org/10.1111/1755-0998.12725

Additionally, please cite the Dryad data package:

Borg Dahl M, Brjenrod AD, Unterseher M, Hoppe T, Feng Y, Novozhilov Y, Sørensen SJ, Schnittler M (2017) Data from: Genetic barcoding of dark-spored myxomycetes (Amoebozoa)—Identification, evaluation and application of a sequence similarity threshold for species differentiation in NGS studies. Dryad Digital Repository. https://doi.org/10.5061/dryad.jv77v
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