Data from: Evolution of ecological dominance of yeast species in high-sugar environments

Williams KM, Liu P, Fay JC

Date Published: July 7, 2015

DOI: http://dx.doi.org/10.5061/dryad.k3f17

 

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Title Pyrosequencing competition data
Downloaded 2 times
Description Calibrated pyrosequencing data from Competition 1. The frequency of each competitor species in either paired competition with S. cerevisiae or in pooled monocultures. The adjusted frequency is based on calibration curves and eliminates negative values.
Download CalibratedPyrosequencingData.csv (14.54 Kb)
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Title Sequencing competition data
Downloaded 3 times
Description Calibrated sequencing data from Competition 2. The frequency of each competitor species in competition with different reference strains, along with concatenated barcode and indexes used for sample ID. The fitness difference and experimental metadata is also included.
Download biodiversity.data.csv (74.06 Kb)
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Title High-sugar environment
Downloaded 4 times
Description Data from assays (gren01 and nut01) to determine the intrinsic growth rate of each yeast species in high-sugar environments including grape juice. The OD600 (cell density) is given for time-points between zero and 48 hrs.
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Title Ethanol resistance
Downloaded 4 times
Description Data from assay to determine the ethanol tolerance of each yeast species. OD600 (cell density) is given for time-points between zero and 48 hrs for different concentrations of ethanol. One sample was omitted from the analysis (Smik, Rep C, 2% ethanol) due to possible contamination from a pipetting error that occurred during the experiment.
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Title Supernatant inhibitors
Downloaded 4 times
Description Data from assay to determine whether Saccharomyces cerevisiae produces unknown compounds that inhibit the growth of other yeast species. OD600 (cell density) is given for for time-points between zero and 48 hrs in supernatant cultures. A small number of samples (24/432) registered growth in un-innoculated controls before 36 hours, and these samples were omitted from our analysis. Following the removal of those samples, Lachancea kluyveri lacked sufficient control samples for analysis and was not included in the analysis.
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Title Sequencing metadata
Downloaded 40 times
Description Barcodes, indexes and metadata used to extract species tags from raw sequencing data for BITS-3 and BITS-4 libraries.
Download Metadata.xls (129.5 Kb)
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Title BITS-3 library read
Downloaded 8 times
Description Raw sequence data with reads containing barcode, linker then species specific sequences.
Download BITS-3_S1_L001_R1_001.fastq.gz (70.76 Mb)
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Title BITS-3 library index
Downloaded 6 times
Description Raw sequence data with reads containing indexes.
Download BITS-3_S1_L001_I1_001.fastq.gz (5.793 Mb)
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Title BITS-4 library read
Downloaded 7 times
Description Raw sequence data with reads containing barcode, linker then species specific sequences.
Download BITS-4_S1_L001_R1_001.fastq.gz (1.115 Gb)
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Title BITS-4 library index
Downloaded 7 times
Description Raw sequence data with reads containing indexes.
Download BITS-4_S1_L001_I1_001.fastq.gz (127.7 Mb)
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When using this data, please cite the original publication:

Williams KM, Liu P, Fay JC (2015) Evolution of ecological dominance of yeast species in high-sugar environments. Evolution 69(8): 2079-2093. http://dx.doi.org/ 10.1111/evo.12707

Additionally, please cite the Dryad data package:

Williams KM, Liu P, Fay JC (2015) Data from: Evolution of ecological dominance of yeast species in high-sugar environments. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.k3f17
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