Background: Stressful life leads to mood disorders. Chronic mild stress is presumably major etiology for depression, and acute severe stress leads to anxiety. These stressful situations may impair hypothalamus-pituitary-adrenal axis and in turn induce synapse dysfunction. However, it remains elusive how the stress hormones mess up subcellular compartments and interactions between excitatory and inhibitory neurons, which we have investigated in mouse amygdala, a structure related to emotional states. Methods and Results: Dexamethasone was chronically given by intraperitoneal injection once a day for one week or was acutely washed into the brain slices. The neuronal spikes and synaptic transmission were recorded by whole-cell patching in amygdala neurons of brain slices. The chronic or acute administration of dexamethasone downregulates glutamate release as well as upregulates GABA release and GABAergic neuron spiking. The chronic administration of dexamethasone also enhances the responsiveness of GABA receptors. Conclusion: The upregulation of GABAergic neurons and the downregulation of glutamatergic neurons by glucocorticoid impair their balance in the amygdala, which leads to emotional disorders during stress.
40 mg/kg DEX injection-sEPSC Ctrl.12 Inj.19
This excel includes all original data related to sEPSC recorded on GABAergic neurons from 40 mg/kg DEX injection and saline injection mice.
injection-sEPSC Ctrl.12 Inj.19.xlsx
40 mg/kg DEX injection-sIPSC Ctrl.13 Inj.11
This excel includes all available original data related to sIPSC recorded on glutamatergic neurons in in the mouse amygdala, in which the mice were treated by the intraperitoneal injections of dexamethasone (DEX,40 mg/kg) once a day or for one week.There are 13 cells in control group and 11 cells in the DEX injection group.
injection-sIPSC Ctrl.13 Inj.11.xlsx
ISI(40 mg/kg DEX injection-GABAergic neurons) Ctrl.11 Inj.11
This excel includes all available original data related to the intervals of spikes(ISI) recorded on GABAergic neurons in DEX injection mice(n=11 cells) and in saline injection mice(n=11 cells)
ISI(injection-IN) Ctrl.11 Inj.11.xlsx
ISI(40 mg/kg DEX injection-Gluergic neurons) Ctrl.19 Inj.28
This excel includes original data related to the inter-spike intervals(ISI) recorded on Gluergic neurons in DEX injection (n=28 cells) and saline injection(n=19 cells).
ISI(injection-PN) Ctrl.19 Inj.28.xlsx
25 uM DEX washing-on sEPSC n=14cells
This excel includes original data related to the inter-spike intervals(ISI) recorded on GABAergic neurons(n=14) before and after DEX washing on.
washing-on EPSC 14cells.xlsx
25 uM DEX washing-on IPSC n=10cells
This excel includes original data related to the inter-spike intervals(ISI) recorded on Gluergic neurons(n=10) before and after DEX washing on.
25 uM DEX washing-on IPSC 10cells.xlsx
ISI(25 uM DEX washing-on-GABAergic neurons)13Cells
This excel includes original data related to the inter-spike intervals(ISI) recorded on GABAergic neurons(n=13) before and after DEX washing on.
ISI(25 uM DEX washing-on-GABAergic neurons) 13cells.xls
ISI (25 uM DEX washing-on-Gluergic neurons) 14cells
This excel includes original data related to the inter-spike intervals recorded on Gluergic neurons(n=14) before and after DEX washing on.
40 mg/kg DEX injectioin-sEPSC Ctrl.12 Inj.19
This is an opj. file which can be opened by origin software.It includes data related to sIPSC on Gluergic neurons from DEX injection and saline injection mice and data processing. Moreover,origin software is used to analyse all data related to our exprements.
40 mg per kg body weight DEX injectioin-sEPSC Ctrl.12 Inj.19.opj
40 mg/kg DEX injection-sIPSC Ctrl.13 Inj.11
This is an opj. file which can be opened by origin software.It includes data related to sIPSC on Gluergic neurons from DEX injection and saline injection mice and data processing.
40 mg per kg DEX injection-sIPSC Ctrl.13 Inj.11.opj
ISI(40 mg/kg DEX injection-GABAergic neuron) Ctrl.11 Inj.11
An opj.file including data related to inter-spike intervals on GABAergic neurons from DEX injection and saline injection mice and data processing.
ISI(DEX injection-GABAergic neuron) Ctrl.11 Inj.11.opj
ISI(40 mg/kg DEX injection-Gluergic neuron) Ctrl.19 Inj. 28
An opj.file including data related to the inter-spike intervals on Gluergic neurons from DEX injection and saline injection and data processing.
ISI(DEX injection-Gluergic neuron ) Ctrl.19 Inj. 28.opj
25 uM DEX washing-on sEPSC 14cells
An opj. file including data related to sEPSC on GABAergic neurons before and after DEX washing on and data processing.
25 uM washing-on sEPSC 14cells.opj
25 uM DEX washing-on-sIPSC 10cells
An opj. file including data related to sIPSC on Gluergic neurons before and after DEX washing on and data processing.
25 uM washing-on-IPSC 10cells.opj
ISI(25 uM DEX washing-on-GABAergic neuron)13Cells
An opj. file including data related to inter-spike intervals(ISI) on GABAergic neurons before and after DEX washing on and data processing.
ISI(25 uM DEX washing-on-GABAergic neurons)13Cells.opj
ISI(25 uM DEX washing-on-Gluergic neuron)14Cells
An opj. file including data related to inter-spike intervals on Gluergic neurons before and after DEX washing on and data processing.