Data from: Metabarcoding for the parallel identification of several hundred predators and their preys: application to bat species diet analysis

Galan M, Pons J, Tournayre O, Pierre E, Leuchtmann M, Pontier D, Charbonnel N

Date Published: December 27, 2017

DOI: https://doi.org/10.5061/dryad.kv02g

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Title MiSeq raw sequences of the COI minibarcode from the faecal pellets of 357 bats from Western France (part 1 to 2)
Downloaded 2 times
Description This ZIP file contains the FASTQ files of the paired-end reads (R1: reads 1; R2: reads 2) produced for each faecal pellet in triplicate using the MiSeq platform. The 1162 multiplexed PCR products were indexed using both forward and reverse indices. The list of the 357 multiplexed samples and the 24 positive and 58 negative controls are provided in the following XLSX file titled: Information concerning the samples multiplexed in the MiSeq Run.
Download MiSeq_Reads_COI_Bat_ faecal_samples_part1.zip (649.5 Mb)
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Title MiSeq raw sequences of the COI minibarcode from the faecal pellets of 357 bats from Western France (part 2 to 2)
Downloaded 3 times
Description This ZIP file contains the FASTQ files of the paired-end reads (R1: reads 1; R2: reads 2) produced for each faecal pellet in triplicate using the MiSeq platform. The 1162 multiplexed PCR products were indexed using both forward and reverse indices. The list of the 357 multiplexed samples and the 24 positive and 58 negative controls are provided in the following XLSX file titled: Information concerning the samples multiplexed in the MiSeq Run.
Download MiSeq_Reads_COI_Bat_ faecal_samples_part2.zip (619.2 Mb)
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Title MiSeq raw sequences of the COI minibarcode from 24 positive controls and 58 negative controls
Downloaded 1 time
Description This ZIP file contains the FASTQ files of the paired-end reads (R1: reads 1; R2: reads 2) produced for each faecal pellet in triplicate using the MiSeq platform. The 1162 multiplexed PCR products were indexed using both forward and reverse indices. The list of the 357 multiplexed samples and the 24 positive and 58 negative controls are provided in the following XLSX file titled: Information concerning the samples multiplexed in the MiSeq Run.
Download MiSeq_Reads_COI_Controls.zip (22.63 Mb)
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Title MiSeq raw sequences of the COI minibarcode from arthropod mock communities MC1 & MC2
Downloaded 1 time
Description This ZIP file contains the FASTQ files of the paired-end reads (R1: reads 1; R2: reads 2) produced using the MiSeq platform for each individual PCR and the pools of the arthropod mock communities in triplicate. The 1162 multiplexed PCR products were indexed using both forward and reverse indices. The list of the 357 multiplexed samples and the 24 positive and 58 negative controls are provided in the following XLSX file titled: Information concerning the samples multiplexed in the MiSeq Run.
Download MiSeq_Reads_COI_Mock_communities.zip (50.21 Mb)
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Title Raw output files generated by the mothur program
Downloaded 1 time
Description This ZIP file contains three output files (.table, .fasta and .log) generated by the mothur program for the whole COI minibarcode dataset. The .log file contains all the mothur command lines used for the processing of the raw sequences.
Download mothur_raw_output_files.zip (432.8 Kb)
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Title Information concerning the samples and positive and negative controls multiplexed in the MiSeq run
Downloaded 1 time
Description This XLSX file contains the sample IDs, the sample types, the PCR IDs, the PCR replicate numbers, the localities, the bat species and the fastq file names for each of the 1162 PCR products multiplexed in the Illumina MiSeq run.
Download Information_concerning_the samples.xlsx (178.9 Kb)
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Title Raw abundance table before filtering
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Description This XLSX file contains the number of reads for each distinct variant of the MiSeq run and for each PCR product before the data filtering process.
Download Table_of_abundance_before_filtering.xlsx (19.93 Mb)
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Title Abundance table after filtering
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Description This XLSX file contains the number of reads for each distinct variant of the MiSeq runs and each sample after the data filtering. The results for the three PCR replicates of a same sample were summed. The sheet "Bat faecal samples" contains the results for the faecal pellets from bats, and the sheet "Mock communities" contains the results for the arthropod mock communities.
Download Table_of_abundance_after_filtering.xlsx (1.186 Mb)
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Title Alignment of 133bp minibarcode COI reference sequences from Barcode of Life Database and corresponding to 33 bat species found in France
Downloaded 3 times
Description This FASTA file contains 444 reference sequences available in BOLD for the 33 bat species found in France. We removed sequences showing less than 132bp for the COI minibarcode used in our study and/or showing one or more ambiguous base (N). Note that the sequences GBMA9839-15, ABBWP228-07, NOMAM103-16, NOMAM142-16 and SKBPA621-11 might not be reliable: they could have been assigned to the wrong species or may be of low quality (see results in the phylogenetic tree and Table S4, Supplemental Information).
Download Alignement_COI_133bp_minibarcode_444_BOLD...ta.txt (75.19 Kb)
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Title Alignment of COI reference sequences available for 33 bat species found in France and recorded in Barcode of Life Database for the 29bp position of the reverse primer
Downloaded 3 times
Description This FASTA file contains 693 reference sequences from BOLD corresponding to the 33 bat species found in France. We removed sequences showing less than 29bp for the position of the reverse primer used in our study and/or showing one or more ambiguous base (N). See also Table S4 for a summary.
Download Alignement_COI_29bp_revers primer_693_BOLD...fasta (43.53 Kb)
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Title Alignment of the COI haplotypes obtained by Sanger and MiSeq sequencing for the 19 insect species included in the mock communities
Downloaded 2 times
Description This FASTA file contains 19 and 20 COI sequences generated by Sanger and Illumina Miseq sequencing respectively. The Sanger sequences correspond to a fragment of 634 to 658bp of the standard COI barcode following the Barcode of Life Consortium. The MiSeq sequences correspond to the 133bp COI minibarcode used in this study. For this latter, only the most abundant variant for each of the 19 samples is provided, except for Forficula lesnei specimen for which an unexpected parasitoid (Triarthria setipennis) was detected and is also provided.
Download Alignment_COI_Sanger_MiSeq_Mock_Communities.fasta (17.51 Kb)
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When using this data, please cite the original publication:

Galan M, Pons J, Tournayre O, Pierre É, Leuchtmann M, Pontier D, Charbonnel N (2017) Metabarcoding for the parallel identification of several hundred predators and their preys: application to bat species diet analysis. Molecular Ecology Resources, online in advance of print. https://doi.org/10.1111/1755-0998.12749

Additionally, please cite the Dryad data package:

Galan M, Pons J, Tournayre O, Pierre E, Leuchtmann M, Pontier D, Charbonnel N (2017) Data from: Metabarcoding for the parallel identification of several hundred predators and their preys: application to bat species diet analysis. Dryad Digital Repository. https://doi.org/10.5061/dryad.kv02g
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