Data from: Assessment of a 16S rRNA amplicon Illumina sequencing procedure for studying the microbiome of a symbiont-rich aphid genus

Jousselin E, Clamens A, Galan M, Bernard M, Maman S, Gschloessl B, Duport M, Meseguer AS, Calevro F, Coeur d'acier A

Date Published: October 8, 2015

DOI: http://dx.doi.org/10.5061/dryad.m8g90

 

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Title Illumina_16SCinara
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Description This is an archive containing results of the sequencing of 16S rRNA gene for all samples of the publication (the results have been demultiplexed : each file corresponds to the sequecining of a PCR product).
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Download Illumina_16SCinara.7z (609.1 Mb)
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Title final.aln.count_table
Downloaded 8 times
Description this compiles the distribution of sequences across samples (this file can be uploaded to run the R script)
Download final.aln.count_table.txt (12.34 Mb)
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Title final.aln.fasta
Downloaded 6 times
Description Final alignment of all unique sequences: output from the Galaxy pipeline, input of the R script
Download final.aln.fasta.fasta (19.18 Mb)
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Title Appendix1_R
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Description R script described in the paper to analyse output from Galaxy -produce summary tables (number of sequences per sample before and after applying various thresholds, distribution of sequences across samples , -scripto to conduct diversity analyses described in the paper
Download Appendix1_R.txt (8.382 Kb)
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When using this data, please cite the original publication:

Jousselin E, Clamens A, Galan M, Bernard M, Maman S, Gschloessl B, Duport M, Meseguer AS, Calevro F, Coeur d'acier A (2015) Assessment of a 16S rRNA amplicon Illumina sequencing procedure for studying the microbiome of a symbiont-rich aphid genus. Molecular Ecology Resources, online in advance of print. http://dx.doi.org/10.1111/1755-0998.12478

Additionally, please cite the Dryad data package:

Jousselin E, Clamens A, Galan M, Bernard M, Maman S, Gschloessl B, Duport M, Meseguer AS, Calevro F, Coeur d'acier A (2015) Data from: Assessment of a 16S rRNA amplicon Illumina sequencing procedure for studying the microbiome of a symbiont-rich aphid genus. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.m8g90
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