Data from: Symbiodinium glynnii sp. nov., a species of stress-tolerant symbiotic dinoflagellates from pocilloporid and montiporid corals in the Pacific Ocean

Wham DC, Ning G, LaJeunesse TC

Date Published: April 5, 2017

DOI: http://dx.doi.org/10.5061/dryad.mg363

 

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Title Pyrenoid_S.glynnii_example1
Downloaded 2 times
Description 3-D movie reconstruction of pyrenoid ultrastructure showing connections (stalks) to the cell cytoplasm and chloroplast lobes. Data were obtained from serial block face scanning electron microscopy on preserved cells of Symbiodinium glynnii sp. nov. Detailed protocol concerning the chemical fixation and preparation of specimens for serial block face scanning electron microscopy available at Deerinck TJ, Bushong EA, Thor A, and Ellisman EH (2010). NCMIR Methods for 3D EM: A New Protocol for Preparation of Biological Specimens for Serial Blockface Scanning Electron Microscopy. SBEM Protocol version 7_01_10. Center for Research in Biological Systems and the National Center for Microscopy and Imaging Research, University of California, San Diego, La Jolla, CA. https://ncmir.ucsd.edu/sbem-protocol
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Title Pyrenoid_S.glynnii_example2
Downloaded 1 time
Description 3-D movie reconstruction of pyrenoid ultrastructure showing connections (stalks) to the cell cytoplasm and chloroplast lobes. Data were obtained from serial block face scanning electron microscopy on preserved cells of Symbiodinium glynnii sp. nov. Detailed protocol concerning the chemical fixation and preparation of specimens for serial block face scanning electron microscopy available at Deerinck TJ, Bushong EA, Thor A, and Ellisman EH (2010). NCMIR Methods for 3D EM: A New Protocol for Preparation of Biological Specimens for Serial Blockface Scanning Electron Microscopy. SBEM Protocol version 7_01_10. Center for Research in Biological Systems and the National Center for Microscopy and Imaging Research, University of California, San Diego, La Jolla, CA. https://ncmir.ucsd.edu/sbem-protocol
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Title Pyrenoid_S.glynnii_example3
Downloaded 4 times
Description 3-D movie reconstruction of pyrenoid ultrastructure showing connections (stalks) to the cell cytoplasm and chloroplast lobes. Data were obtained from serial block face scanning electron microscopy on preserved cells of Symbiodinium glynnii sp. nov. Detailed protocol concerning the chemical fixation and preparation of specimens for serial block face scanning electron microscopy available at Deerinck TJ, Bushong EA, Thor A, and Ellisman EH (2010). NCMIR Methods for 3D EM: A New Protocol for Preparation of Biological Specimens for Serial Blockface Scanning Electron Microscopy. SBEM Protocol version 7_01_10. Center for Research in Biological Systems and the National Center for Microscopy and Imaging Research, University of California, San Diego, La Jolla, CA. https://ncmir.ucsd.edu/sbem-protocol
Download Pyrenoid_S.glynnii_example3.avi (672.3 Kb)
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Title Pyrenoid_S.glynnii_example4
Downloaded 1 time
Description 3-D movie reconstruction of pyrenoid ultrastructure showing connections (stalks) to the cell cytoplasm and chloroplast lobes. Data were obtained from serial block face scanning electron microscopy on preserved cells of Symbiodinium glynnii sp. nov. Detailed protocol concerning the chemical fixation and preparation of specimens for serial block face scanning electron microscopy available at Deerinck TJ, Bushong EA, Thor A, and Ellisman EH (2010). NCMIR Methods for 3D EM: A New Protocol for Preparation of Biological Specimens for Serial Blockface Scanning Electron Microscopy. SBEM Protocol version 7_01_10. Center for Research in Biological Systems and the National Center for Microscopy and Imaging Research, University of California, San Diego, La Jolla, CA. https://ncmir.ucsd.edu/sbem-protocol
Download Pyrenoid_S.glynnii_example4.avi (714.4 Kb)
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Title Pyrenoid_S.glynnii_example5
Downloaded 4 times
Description 3-D movie reconstruction of pyrenoid ultrastructure showing connections (stalks) to the cell cytoplasm and chloroplast lobes. Data were obtained from serial block face scanning electron microscopy on preserved cells of Symbiodinium glynnii sp. nov. Detailed protocol concerning the chemical fixation and preparation of specimens for serial block face scanning electron microscopy available at Deerinck TJ, Bushong EA, Thor A, and Ellisman EH (2010). NCMIR Methods for 3D EM: A New Protocol for Preparation of Biological Specimens for Serial Blockface Scanning Electron Microscopy. SBEM Protocol version 7_01_10. Center for Research in Biological Systems and the National Center for Microscopy and Imaging Research, University of California, San Diego, La Jolla, CA. https://ncmir.ucsd.edu/sbem-protocol
Download Pyrenoid_S.glynnii_example5.avi (708.3 Kb)
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Title Pyrenoid_S.glynnii_example6
Downloaded 2 times
Description 3-D movie reconstruction of pyrenoid ultrastructure showing connections (stalks) to the cell cytoplasm and chloroplast lobes. Data were obtained from serial block face scanning electron microscopy on preserved cells of Symbiodinium glynnii sp. nov. Detailed protocol concerning the chemical fixation and preparation of specimens for serial block face scanning electron microscopy available at Deerinck TJ, Bushong EA, Thor A, and Ellisman EH (2010). NCMIR Methods for 3D EM: A New Protocol for Preparation of Biological Specimens for Serial Blockface Scanning Electron Microscopy. SBEM Protocol version 7_01_10. Center for Research in Biological Systems and the National Center for Microscopy and Imaging Research, University of California, San Diego, La Jolla, CA. https://ncmir.ucsd.edu/sbem-protocol
Download Pyrenoid_S.glynnii_example6.avi (1.819 Mb)
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Title Pyrenoid_S.glynnii_example7
Downloaded 1 time
Description 3-D movie reconstruction of pyrenoid ultrastructure showing connections (stalks) to the cell cytoplasm and chloroplast lobes. Data were obtained from serial block face scanning electron microscopy on preserved cells of Symbiodinium glynnii sp. nov. Detailed protocol concerning the chemical fixation and preparation of specimens for serial block face scanning electron microscopy available at Deerinck TJ, Bushong EA, Thor A, and Ellisman EH (2010). NCMIR Methods for 3D EM: A New Protocol for Preparation of Biological Specimens for Serial Blockface Scanning Electron Microscopy. SBEM Protocol version 7_01_10. Center for Research in Biological Systems and the National Center for Microscopy and Imaging Research, University of California, San Diego, La Jolla, CA. https://ncmir.ucsd.edu/sbem-protocol
Download Pyrenoid_S.glynnii_example7.avi (1.488 Mb)
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Title psbA coding and non-coding region (partial sequences)
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Description Sequence alignments of partial psbA coding and non-coding region haplotypes of Symbiodinium glynnii sp. nov. from the western, central and eastern Pacific Ocean.
Download PsbA_trenchii_vs_glynni_DryadFile (55.17 Kb)
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Title Cell1_Pal16_1_ROI_02.1a
Downloaded 8 times
Description Serial sections and chromosome counts of the cell nucleus from Symbiodinium glynnii sp. nov.
Download Cell1_Pal16_1_ROI_02.1a.jpg (3.387 Mb)
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Title Cell1_Pal16_1_ROI_02.1b
Downloaded 4 times
Description Serial sections and chromosome counts of the cell nucleus from Symbiodinium glynnii sp. nov.
Download Cell1_Pal16_1_ROI_02.1b.jpg (3.567 Mb)
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Title Cell1_Pal16_1_ROI_02.1c
Downloaded 8 times
Description Serial sections and chromosome counts of the cell nucleus from Symbiodinium glynnii sp. nov.
Download Cell1_Pal16_1_ROI_02.1c.jpg (3.564 Mb)
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Title Cell2_Pal16_1-2_sept19b
Downloaded 9 times
Description Serial sections and chromosome counts of the cell nucleus from Symbiodinium glynnii sp. nov.
Download Cell2_Pal16_1-2_sept19b.jpg (2.216 Mb)
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Title Cell2_Pal16-1-2_Sept19a
Downloaded 8 times
Description Serial sections and chromosome counts of the cell nucleus from Symbiodinium glynnii sp. nov.
Download Cell2_Pal16-1-2_Sept19a.jpg (2.661 Mb)
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Title Cell3_Chromosome_counts_S.glynnii
Downloaded 9 times
Description Serial sections and chromosome counts of the cell nucleus from Symbiodinium glynnii sp. nov.
Download Cell3_Chromosome_counts_S.glynnii.jpg (2.615 Mb)
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Title Cell4_Pal16-1-2-sept19-cell3
Downloaded 5 times
Description Serial sections and chromosome counts of the cell nucleus from Symbiodinium glynnii sp. nov.
Download Cell4_Pal16-1-2-sept19-cell3.jpg (2.893 Mb)
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Title Cell5_Pal16_1_glynnii_ROI_01a
Downloaded 7 times
Description Serial sections and chromosome counts of the cell nucleus from Symbiodinium glynnii sp. nov.
Download Cell5_Pal16_1_glynnii_ROI_01a.jpg (3.201 Mb)
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Title Cell5_Pal16_1_glynnii_ROI_01b
Downloaded 4 times
Description Serial sections and chromosome counts of the cell nucleus from Symbiodinium glynnii sp. nov.
Download Cell5_Pal16_1_glynnii_ROI_01b.jpg (2.883 Mb)
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Title Cell5_Pal16_1_glynnii_ROI_01c
Downloaded 6 times
Description Serial sections and chromosome counts of the cell nucleus from Symbiodinium glynnii sp. nov.
Download Cell5_Pal16_1_glynnii_ROI_01c.jpg (2.959 Mb)
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Title Cell5_Pal16_1_glynnii_ROI_01d
Downloaded 7 times
Description Serial sections and chromosome counts of the cell nucleus from Symbiodinium glynnii sp. nov.
Download Cell5_Pal16_1_glynnii_ROI_01d.jpg (2.893 Mb)
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Title SymbXGb.r
Downloaded 3 times
Description This script creates a classification/regression model then validates the model using a subset of samples where data is complete for all character traits.
Download SymbXGb.r (3.154 Kb)
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Title Master microsatellite Dataset
Downloaded 4 times
Description Master file of microsatellite data, including host identity and location information, Symbiodinium species, ITS2 genotype
Download Master microsatellite Dataset.xlsx (126.6 Kb)
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Title makeTrainTest
Downloaded 1 time
Description R script that reads in all data sources, merges them into a single master data table, and then divides the data into a training and test set
Download makeTrainTest.r (2.866 Kb)
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When using this data, please cite the original publication:

Wham DC, Ning G, LaJeunesse TC (2017) Symbiodinium glynnii sp. nov., a species of stress-tolerant symbiotic dinoflagellates from pocilloporid and montiporid corals in the Pacific Ocean. Phycologia 56(4): 396-409. http://dx.doi.org/10.2216/16-86.1

Additionally, please cite the Dryad data package:

Wham DC, Ning G, LaJeunesse TC (2017) Data from: Symbiodinium glynnii sp. nov., a species of stress-tolerant symbiotic dinoflagellates from pocilloporid and montiporid corals in the Pacific Ocean. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.mg363
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