Data from: Influence of factors of cryopreservation and hypothermic storage on survival and functional parameters of multipotent stromal cells of placental origin

Pogozhykh D, Prokopyuk V, Pogozhykh O, Mueller T, Prokopyuk O

Date Published: October 8, 2015

DOI: http://dx.doi.org/10.5061/dryad.mk556

 

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Title Fig 1. Characterization of primary cell culture derived from placenta for MSC origin.
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Description (A) RT-PCR analysis for expression of typical MSC markers: cells provide a signal for CD90, CD73, and CD105 MSC while being negative for CD34 hematopoietic marker. (B) Minus RT control for RT-PCR. (C,D) Cells display strong signals for CD73 and CD105 in flow cytometry analysis.
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Title Fig 2. Differentiation potential of primary cell culture derived from placenta into lineages of MSC origin.
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Description MSCs were differentiated into (A) adipogenic (Oil Red O staining), (B) osteogenic (Von Kossa staining), and (C) chondrogenic (Alcian blue staining) lineages as a proof of principle for plasticity of MSCs. Cells expanded in regular MSC culture medium represent the negative control (bar = 50 µm).
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Title Fig 3. MSCs of placenta after storage at +20°C (A,B) and 4°C (C,D).
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Description Trypan blue staining, (A,C); phase contrast microscopy (B,D). Scale bar is 200 µm.
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Title Fig. 4. Parameters of placental MSCs after cryopreservation with different cooling rates.
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Description (A) - cell number in the sample; (B) - viable cells according to Trypan blue and 7 AAD staining; (C) - functional activity of cells in studied samples; (D) – adherence to plastic. *- significance to positive control p<0.05.
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Title Fig. 4. Parameters of placental MSCs after cryopreservation with different cooling rates:
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Description Data set for Fig.4
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Title Table 1. Characteristics of placental MSCs after short-term storage at different temperature conditions
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Description Data set for Table 1
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Title Table 2. Cryopreservation of placental MSCs with various CPAs.
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Description Data set for Table 2
Download PLOS ONE Pogozhykh (Table_2).xls (37.37 Kb)
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Title Table 3. Cryopreservation of placental MSCs with various cryoprotective media.
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Description Data set for Table 3
Download PLOS ONE Pogozhykh (Table_3).xls (39.42 Kb)
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When using this data, please cite the original publication:

Pogozhykh D, Prokopyuk V, Pogozhykh O, Mueller T, Prokopyuk O (2015) Influence of factors of cryopreservation and hypothermic storage on survival and functional parameters of multipotent stromal cells of placental origin. PLOS ONE 10(10): e0139834. http://dx.doi.org/10.1371/journal.pone.0139834

Additionally, please cite the Dryad data package:

Pogozhykh D, Prokopyuk V, Pogozhykh O, Mueller T, Prokopyuk O (2015) Data from: Influence of factors of cryopreservation and hypothermic storage on survival and functional parameters of multipotent stromal cells of placental origin. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.mk556
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