Data from: Factors influencing detection of eDNA from a stream-dwelling amphibian

 

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Title Pilliod_et_al_2013_MER_DryadData_7-31-13
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Description We handled, stored, and extracted eDNA from 0.45 nm Cellulose Nitrate filter paper the same way for each experiment using protocols described in Pilliod et al. (2013). We extracted DNA from half of each filter paper using the Qiashredder/DNeasy Blood & Tissue DNA extraction kit method described in Goldberg et al. (2011). We used the quantitative PCR assay described in Pilliod et al. (2013) to estimate the amount of eDNA in each sample. We used the QuantiTect Multiplex PCR Mix (Qiagen, Inc., Gaithersburg, Maryland, USA) with recommended multiplexing concentrations and parameters on an Applied Biosystems 7500 Fast Real-Time PCR System to conduct the assay. We used 2 µL of DNA extract in each reaction and ran all reactions in triplicate. Standard curves were constructed from whole genomic DNA extracted from tail tissue and diluted to 0.001, 0.01, and 0.1 ng. Average r2 for curves used in this study was 0.99. We considered a test result negative if there was no exponential phase at any point during 50 cycles. See Pilliod et al. (2013) for additional methodological details.
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When using this data, please cite the original publication:

Pilliod DS, Goldberg CS, Arkle RS, Waits LP (2014) Factors influencing detection of eDNA from a stream-dwelling amphibian. Molecular Ecology Resources 14(1): 109-116. http://dx.doi.org/10.1111/1755-0998.12159

Additionally, please cite the Dryad data package:

Pilliod DS, Goldberg CS, Arkle RS, Waits LP (2013) Data from: Factors influencing detection of eDNA from a stream-dwelling amphibian. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.mt60p
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