Data from: Optogenetic recruitment of dorsal raphe serotonergic neurons acutely decreases mechanosensory responsivity in behaving mice

Dugué GP, Lörincz ML, Lottem E, Audero E, Matias S, Correia PA, Léna C, Mainen ZF

Date Published: September 3, 2014

DOI: http://dx.doi.org/10.5061/dryad.nh437

 

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Title Spike probability vs irradiance
Description Experiment: photosensitivity of ChR2-EYFP-positive dorsal raphe neurons assessed in acute slices (see the Supplementary Information section for a detailed description of the protocol). The file contains the average spike probabilities of 21 neurons for different irradiance values (Fig. 1H and I in the paper). Columns with names ending with "_SpikeProb" contain the spike probabilities. Columns with names ending with "_Irradiance" contain irradiance values in mW/mm2. E.g., for cell 5, the spike probability curve can be obtained by plotting "Cell5_SpikeProb" versus "Cell5_Irradiance".
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Title Spike times at twice the photostimulation threshold
Description Experiment: Spiking reliability of ChR2-EYFP-positive dorsal raphe neurons in response to trains of light pulses at different frequencies, using an irradiance = twice the photostimulation threshold. This zip file contains a list of 30 .csv file. Each .csv file contains a table of spike times (time of occurrence of spikes, in seconds) measured for one neuron in response to trains of light pulses at different frequencies (1, 2, 5, 10, 20 and 50 Hz). Each column represents one frequency (the frequency is indicated in the first row). Columns are filled with NAs at the end so that they have the same length. These data were used to generate the plots in Fig. 1L, Supp. Fig. S1B and Fig. S1D1 to D3.
Download Spike_times_2X_photostimulation_threshold.zip (120.5 Kb)
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Title Spike times at maximal irradiance
Description Experiment: Spiking reliability of ChR2-EYFP-positive dorsal raphe neurons in response to trains of light pulses at different frequencies, using an irradiance = ~5 mW/mm2. This zip file contains a list of 14 .csv file. Each .csv file contains a table of spike times (time of occurrence of spikes, in seconds) measured for one neuron in response to trains of light pulses at different frequencies (1, 2, 5, 10, 20 and 50 Hz). Each column represents one frequency (the frequency is indicated in the first row). Columns are filled with NAs at the end so that they have the same length. These data were used to generate the plots in Fig. 1L, Supp. Fig. S1B and Fig. S1E1 to E3.
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Title Number of spikes vs pulse duration
Description Experiment: assessing the number of spikes evoked in ChR2-EYFP-positive dorsal raphe neurons, for light pulses of various duration. This .csv contains the average number of spikes obtained in individual neurons in response to light pulses of different durations (0.1 to 1000 ms; n = 17 neurons). Each row corresponds to one neuron. Each column corresponds to one pulse duration (the duration is indicated in the first row). These data were used to generated Fig. 1J.
Download Number of spikes vs pulse duration.csv (591 bytes)
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Title Light transmission in DRN
Description This .zip file contains pictures obtained by shining light through an optical fiber over the dorsal aspect of the dorsal raphe nucleus (in freshly dissected brain tissue, see the paper for a detailed description of the procedure). The archive contains pictures from 3 different brains. For each brain, the same field of view was imaged using brightfield illumination (file names ending with "_TL") in order to show the contours of the DRN, and under illumination through the optical fiber (file names ending with "_OF_ILLUMINATION"). The format is 12 bits encoding. These pictures were used to generate Fig. 2B, C and D.
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Title Linear pixel intensity profiles
Description Experiment: light propagation in the dorsal raphe nucleus. This .csv file contains normalized pixel intensity profiles for 3 brains, measured along the optical fiber axis (see paper for a detailed description of the procedure). These data are plotted in Fig. 1D.
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Title Combined fluorescence and OLFP mapping
Downloaded 1 time
Description Experiment: combined fluorescence and OLFP (= optically-evoked field potential) mapping in vivo. This file contains the following values for 8 different animals: average fluorescence intensity in the first millimeter of tissue, maximal fluorescence intensity, position of point of maximal fluorescence, full width at half maximum of fluorescence intensity profile, maximal value of OLFP amplitude, position of point with largest OLFP and full width at half maximum of OLFP amplitude profile (see README.txt). These data were used in Fig. 2F.
Download Combined fluorescence and OLFP mapping.csv (532 bytes)
Download README.txt (684 bytes)
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Title Effet of 8-OHDPAT on OLFP
Description Experiment: sensitivity of the optically-evoked field potential (OLFP) to a 5-HT1A receptor agonist (8-OH-DPAT). This file contains the average OLFP amplitude and peak latency before and after the injection of 8-OH-DPAT (see the paper for a detailed description of the protocol) for 10 mice. These data were used in Fig. 2H.
Download Effet_of_8-OHDPAT_on_OLFP.csv (531 bytes)
Download README.txt (355 bytes)
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Title Fits OLFP amplitude and latency vs power
Description This file contains the fit parameters of the OLFP amplitude vs power and OLFP latency vs power curves for 9 mice. See README.txt for a detailed description of the fit parameters.
Download Fits_OLFP_amplitude_and_latency_vs_power.csv (1.014 Kb)
Download README.txt (287 bytes)
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Title OLFP amplitude and latency vs frequency
Description The .zip file contains the values of the OLFP amplitude and latency for different photostimulation frequencies in 7 mice (see the paper for a detailed description of the protocol). The files ending with "_Freq" contain the list of frequencies (in Hz). The files ending with "_Peak_vs_Freq" contain the values of the OLFP amplitude (in Volts). The files ending with "_Lat_vs_Freq" contain the values of the OLFP latency (in seconds). These data were used in Fig. 2J.
Download OLFP_amplitude_and_latency_vs_frequency.zip (4.391 Kb)
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Title OLFP recovery
Description The .zip file contains the values for the OLFP amplitude recovery experiment (Fig. 2K; see the paper and the supporting information for a detailed description of the protocol). The files ending with "_stim_Y" contain the normalized OLFP amplitude values in the stimulation period (in blue on Fig. 2K). The files ending with "_recov_Y" contain the normalized OLFP amplitude values in the recovery period (in green on Fig. 2K). The file "Stim_X.txt" contains the time points for the stimulation period. The file "Recov_X.txt" contains the time points for the recovery period.
Download OLFP_recovery.zip (2.952 Kb)
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Title Von Frey experiment
Description This .csv file contains the scored data for the Von Frey experiment (Fig. 3). See the paper and supporting information for a detailed description of the experiment. A description of the data contained in the .csv is available in the README.txt file.
Download Von_Frey_experiment.csv (185.9 Kb)
Download README.txt (917 bytes)
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When using this data, please cite the original publication:

Dugué GP, Lörincz ML, Lottem E, Audero E, Matias S, Correia PA, Léna C, Mainen ZF (2014) Optogenetic recruitment of dorsal raphe serotonergic neurons acutely decreases mechanosensory responsivity in behaving mice. PLoS ONE 9(8): e105941. http://dx.doi.org/10.1371/journal.pone.0105941

Additionally, please cite the Dryad data package:

Dugué GP, Lörincz ML, Lottem E, Audero E, Matias S, Correia PA, Léna C, Mainen ZF (2014) Data from: Optogenetic recruitment of dorsal raphe serotonergic neurons acutely decreases mechanosensory responsivity in behaving mice. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.nh437
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