Data from: Assessing strengths and weaknesses of DNA metabarcoding based macroinvertebrate identification for routine stream monitoring

Elbrecht V, Vamos EE, Meissner K, Aroviita J, Leese F

Date Published: May 17, 2017

DOI: http://dx.doi.org/10.5061/dryad.nm3pq

 

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Title Metabarcoding pipeline and scripts
Downloaded 110 times
Description This folder contains all scripts used to process the raw sequence data into OTUs. Additionally, all scripts for data analysis and figure generation were included.
Download Dryad_Finland_2017.zip (60.19 Mb)
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Title DNA extraction and PCR protocols
Downloaded 7 times
Description Raw data on laboratory protocols. Includes gel pictures, PCR sheets and scans of the laboratory protocols as well as pictures of the dried samples.
Download laboratory notes.zip (445.0 Mb)
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Title Preprocessed HTS data (144 samples)
Downloaded 6 times
Description Sequence data for the 144 sequenced PCR reactions. Files are PE merged, trimmed and quality filtered as described in the manuscript using the scripts also provided on dryad. The sequence files are dereplicated ready to be clustered into OTUs. For OTU sequences and counts see table S4 of the manuscript.
Download 6 dereplicate.zip (507.2 Mb)
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When using this data, please cite the original publication:

Elbrecht V, Vamos EE, Meissner K, Aroviita J, Leese F (2017) Assessing strengths and weaknesses of DNA metabarcoding-based macroinvertebrate identification for routine stream monitoring. Methods in Ecology and Evolution, online in advance of print. http://dx.doi.org/10.1111/2041-210x.12789

Additionally, please cite the Dryad data package:

Elbrecht V, Vamos EE, Meissner K, Aroviita J, Leese F (2017) Data from: Assessing strengths and weaknesses of DNA metabarcoding based macroinvertebrate identification for routine stream monitoring. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.nm3pq
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