Data from: PTEN controls glandular morphogenesis through a juxtamembrane β-Arrestin1/ARHGAP21 scaffolding complex

Javadi A, Deevi RK, Evergren E, Blondel-Tepaz E, Baillie GS, Scott MGH, Campbell FC

Date Published: August 17, 2017

DOI: https://doi.org/10.5061/dryad.ns5qs

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Title Figure 1B Membrane ARHGAP10 and β-Arrestin1 [ADU]). Experiments in triplicate.
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Description Data for Figure 1B in Excel file. Membrane ARHGAP10 and β-Arrestin1 [ADU]). Experiments in triplicate.
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Title Figure 1D Membrane β-Arrestin1 intensity
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Description Data for Fig 1D in Excel file. Membrane β-Arrestin1 intensity (AI) in Caco-2 and Caco-2 ShPTEN cell monolayers after control or LPA treatment. Experiments in triplicate. In each replicate, signal intensity was assessed in 10 cells (C-L). Mean ± sem values were plotted.
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Title Figure 1F β-Arrestin1 membrane (ADU) in Caco-2 and Caco-2 ShPTEN cells after control or LPA treatment
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Description Data for Fig 1F in Excel file. β-Arrestin1 membrane (ADU) in Caco-2 and Caco-2 ShPTEN cells after control or LPA treatment. Experiments in triplicate.
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Title Figure 1 supplement 2
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Description Data for Fig 1 Supplement 2 in Excel file. Arbitrary intensity values for membrane β-Arrestin1 after LPA treatment in PTEN+/+ and PTEN-/- HCT116 cell monolayers. Confocal imaging conducted in 10 cells for each replicate (n=3) for each experimental condition.
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Title Figure 1 supplement 4
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Description Data for Figure 1 Supplement 4 in Excel file. Membrane β-Arrestin1 ADU in PTEN+/+ and PTEN-/- HCT116 cells after LPA treatment. Experiments in triplicate
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Title Figure 2B
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Description Data for Figure 2B in Excel file. CDC42-GTP ADU in Caco-2 cells after β-Arrestin1 or NT SiRNA. Experiments in triplicate.
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Title Figure 2D
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Description Data for Figure 2D in Excel file. CDC42-GTP (ADU) in Caco-2ShPTEN cells after ARHGAP10 or NT SiRNA transfections. Experiments in triplicate.
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Title Figure 2 supplement 1
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Description Data for Figure 2 Supplement 1 in Excel file. CDC42-GTP ADU in PTEN+/+ cells after NT or β-Arrestin SiRNA transfection. Experiments conducted in triplicate.
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Title Figure 2 supplement 2
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Description Data for Figure 2 Supplement 2 in Excel file. CDC42-GTP ADU in PTEN-/- HCT116 cells after NT or ARHGAP SiRNA transfections. Experiments conducted in triplicate.
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Title Figure 2 supplement 3
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Description Data for Figure 2 Supplement 3 in Excel file. CDC42-GTP intensity (AI) in Caco-2 glands. Intensity was assessed in 10 randomly selected glands for each replicate (n=3) for each experimental condition.
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Title Figure 2 supplement 4
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Description Data for Figure 2 supplement 4 in Excel file. Spindle angles in Caco-2 cells after NT or β-Arrestin1 SiRNA knock down. Spindle angles of 15 mitotic figures assessed for each experimental condition
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Title Figure 2 supplement 5
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Description Data for Figure 2 supplement 5 in Excel file. Caco-2 glands with single lumens after transfection. Assays in 50 glands per replicate (N=3). Results expressed as a percentage.
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Title Figure 2 supplement 6
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Description Data for Figure 2 supplement 6 in Excel file. CDC42-GTP AI in Caco-2 ShPTEN glands. Assays in 10 glands for each replicate (n=3) in each experimental condition
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Title Figure 2 supplement 7
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Description Data for Figure 2 Supplement 7 in Excel file. Spindle angles in Caco-2 ShPTEN cells after NT or ARHGAP10 SiRNA transfection. Assays in 15 glands for each experimental condition.
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Title Figure 2 supplement 8
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Description Data for Figure 2 supplement 8 in Excel file. Caco-2ShPTEN glands with single central lumens after transfection. 50 glands counted per replicate (n=3). Those with single lumens expressed as a percentage.
Download figure 2 supplement 8.xlsx (8.678 Kb)
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Title Figure 3B
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Description Data for Figure 3B in Excel file. β-Arrestin1:ARHGAP binding ADU in Caco-2 or Caco-2 ShPTEN cells. Experiments in triplicate.
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Title Figure 3C
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Description Data for Figure 3C in Excel file. β-Arrestin1:ARHGAP binding ADU in PTEN+/+ and PTEN-/- HCT116 cells
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Title Figure 3E
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Description Data for Figure 3E in Excel file. β-Arrestin1:ARHGAP10 binding ADU in Caco-2 ShPTEN cells after transfection. Experiments in triplicate.
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Title Figure 3G
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Description Data for Figure 3G in Excel file. β-Arrestin1:ARHGAP10 binding ADU in PTEN-/- HCT116 cells. Experiments in triplicate.
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Title Figure 4B
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Description Data for Figure 4B in Excel file. Membrane β-Arrestin1 (ADU) in Caco-2ShPTEN cells after transfection. Experiments in triplicate.
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Title Figure 4C
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Description Data for Figure 4C. Membrane ARHGAP10 ADU in Caco-2ShPTEN cells. Experiments in triplicate
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Title Figure 4E
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Description Data for Figure 4E in Excel file. Membrane β-Arrestin1 (ADU) in PTEN-/-HCT116 cells after transfection. Experiments in triplicate.
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Title Figure 4F
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Description Data for Figure 4F in Excel file. Membrane ARHGAP10 (ADU) in PTEN-/-HCT116 cells. Experiments in triplicate.
Download figure 4f.xlsx (8.787 Kb)
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Title Figure 4H
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Description Data for Figure 4H in Excel file. Membrane β-Arrestin1:ARHGAP10 binding (ADU) in Caco-2 ShPTEN cells after transfection. Experiments in triplicate.
Download figure 4h.xlsx (8.784 Kb)
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Title Figure 4J
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Description Data for Figure 4J in Excel file.Membrane β-Arrestin1:ARHGAP10 binding ADU in PTEN-/-HCT116 cells. Experiments in triplicate
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Title Figure 5B
Downloaded 11 times
Description Data for Figure 5B in Excel file. Membrane β-Arrestin1 intensity (AI) in Caco-2 ShPTEN glands after transfection. Intensity assessed in 10 glands for each replicate (n=3) for each transfection.
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Title Figure 5D
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Description Data for Figure 5D in Excel file. Membrane ARHGAP10 intensity (AI) in Caco-2 ShPTEN glands after transfection. Intensity assessed in 10 glands for each replicate (n=3) for each transfection.
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Title FIGURE 5F
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Description Data for Figure 5F in Excel file. Spindle angle in Caco-2 ShPTEN glands after transfection with EV or C2 or MCBR3 constructs. Spinlde angles of 15 mitotic figures per transfection.
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Title Figure 5 Supplement 2
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Description Data for Figure 5 Supplement 2 in Excel file. Caco-2 ShPTEN glands with single central lumens. Lumens counted in 50 glands and edpressed as a percentage
Download figure 5 supplement 2.xlsx (8.721 Kb)
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Title Figure 6B
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Description Data for Figure 6B in Excel file. β-Arrestin1:ARHGAP10 binding ADU in Caco-2 cells after treatment. Experiments in triplicate.
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Title Figure 6D
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Description Data for Figure 6D in Excel file. CDC42-GTP ADU in Caco-2 cells after treatment. Experiments in triplicate.
Download figure 6d.xlsx (9.563 Kb)
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Title Figure 6F
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Description Data for Figure 6F in Excel file. β-Arrestin1:ARHGAP10 binding ADU in PTEN+/+ HCT116 cells after treatment. Experiments in triplicate.
Download figure 6f.xlsx (9.691 Kb)
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Title Figure 6H
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Description Data for Figure 6H in Excel file. CDC42-GTP (ADU) in PTEN+/+ HCT116 cells after treatment. Experiments in triplicate.
Download figure 6h.xlsx (9.692 Kb)
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Title Figure 6J
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Description Data for Figure 6J in Excel file. Spindle angles in Caco-2 glands after treatment. Spindle angles calculated for 15 mitotic figures per treatment.
Download Figure 6J.xlsx (9.669 Kb)
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Title Figure 6L
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Description Data for Figure 6L in Excel file. Caco-2 glands with single central lumens after treatment. Lumens assessed in 50 glands and expressed as a percentage
Download figure 6L.xlsx (10.17 Kb)
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Title Figure 7B
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Description Data for Figure 7B in Excel file. Spindle angles in Organoids after treatment. Spindle angles calculated from 30 mitotic figures per treatment.
Download Figure 7b.xlsx (9.611 Kb)
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Title Figure 7C
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Description Data for Figure 7C in Excel file. Organoids with single central lumen after treatment. In each replicate (n=3), in each experimental condition 10 organoids were studied and the number with single lumens were expressed as a percentage (%).
Download Figure 7c.xlsx (8.757 Kb)
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When using this data, please cite the original publication:

Javadi A, Deevi RK, Evergren E, Blondel-Tepaz E, Baillie GS, Scott MGH, Campbell FC (2017) PTEN controls glandular morphogenesis through a juxtamembrane β-Arrestin1/ARHGAP21 scaffolding complex. eLife 6: e24578. https://doi.org/10.7554/elife.24578

Additionally, please cite the Dryad data package:

Javadi A, Deevi RK, Evergren E, Blondel-Tepaz E, Baillie GS, Scott MGH, Campbell FC (2017) Data from: PTEN controls glandular morphogenesis through a juxtamembrane β-Arrestin1/ARHGAP21 scaffolding complex. Dryad Digital Repository. https://doi.org/10.5061/dryad.ns5qs
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