Data from: The molecular evolution of spiggin nesting glue in sticklebacks

Seear PJ, Rosato E, Goodall-Copestake WP, Barber I

Date Published: August 11, 2015

DOI: http://dx.doi.org/10.5061/dryad.pc5n9.2

 

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Title Spiggin EST alignment for internal RACE-PCR primer design
Downloaded 6 times
Description A nucleotide alignment of spiggin ESTs generated in an earlier study (Seear et al, 2014, Ecology and Evolution 4(8) 1233-1242) used to design internal reverse RACE-PCR primers.
Download Spiggin EST alignment for internal RACE-PC...fasta (39.59 Kb)
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Title Intrachromosomal spiggin alignment for verification PCR
Downloaded 5 times
Description A nucleotide alignment of spiggin B/C1 and spiggin B/C2 intrachromosomal genes used to design additional internal reverse primers to verify the original intrachromosomal PCR products.
Download Intrachromosomal spiggin alignment for ver...fasta (63.07 Kb)
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Title Full spiggin gene alignment
Downloaded 8 times
Description A nucleotide alignment of all unique spiggin sequences produced in this study with spiggin sequences from the public domain (including all published spiggin cDNAs and representatives from each of the six Ensembl gene predictions from the 2006 draft assembly of G. aculeatus).
Download Full spiggin gene alignment.fasta (574.6 Kb)
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Title Working alignment of accessions from tables S2 and S3
Downloaded 6 times
Description Working alignment of MUC19/Spiggin sequence accessions listed in tables S2 and S3 from Seear et al. Molecular Ecology 2015, with an additional line for excluded/alignment ambiguous regions; note that AB243103 has been duplicated for comparison of both forms of spiggin in this accession.
Download working_alignment_of_accessions_from_tabl...S3.nex (1.889 Mb)
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When using this data, please cite the original publication:

Seear PJ, Rosato E, Goodall-Copestake WP, Barber I (2015) The molecular evolution of spiggin nesting glue in sticklebacks. Molecular Ecology 24(17): 4474–4488. http://dx.doi.org/10.1111/mec.13317

Additionally, please cite the Dryad data package:

Seear PJ, Rosato E, Goodall-Copestake WP, Barber I (2015) Data from: The molecular evolution of spiggin nesting glue in sticklebacks. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.pc5n9.2
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