Data from: Responses of neurogenesis and neuroplasticity related genes to elevated CO2 levels in the brain of three teleost species

Lai F, Fagernes CE, Bernier NJ, Miller GM, Munday PL, Jutfelt F, Nilsson GE

Date Published: August 9, 2017

DOI: https://doi.org/10.5061/dryad.pn397

Files in this package

Content in the Dryad Digital Repository is offered "as is." By downloading files, you agree to the Dryad Terms of Service. To the extent possible under law, the authors have waived all copyright and related or neighboring rights to this data. CC0 (opens a new window) Open Data (opens a new window)

Title Lai et al., 2017_DataSets_Biology letter
Downloaded 3 times
Description Cp values and priming efficiencies were calculated using the second derivative maximum method (Roche Lightcycler 480; Rasmussen, 2001) and the LinRegPCR software (Ruijter et al., 2009), respectively. Subsequently, relative mRNA expression levels were calculated using the following formula: Expression of target gene = (Ega^Cpga/Etar^Cptar) Where ga is the geometric average of the two reference genes; tar is the target gene of interest, E is priming efficiency and Cp is the crossing point. Since duplicate cDNA syntheses were performed, and each of these were analysed in duplicates in the qPCR analyses, four data points were present for each original sample for each primer pair used, and their means were used in the mRNA expression calculations.
Download Lai et al., 2017_DataSets_Biology letter.xlsx (222.1 Kb)
Details View File Details

When using this data, please cite the original publication:

Lai F, Fagernes CE, Bernier NJ, Miller GM, Munday PL, Jutfelt F, Nilsson GE (2017) Responses of neurogenesis and neuroplasticity related genes to elevated CO 2 levels in the brain of three teleost species. Biology Letters 13(8): 20170240. https://doi.org/10.1098/rsbl.2017.0240

Additionally, please cite the Dryad data package:

Lai F, Fagernes CE, Bernier NJ, Miller GM, Munday PL, Jutfelt F, Nilsson GE (2017) Data from: Responses of neurogenesis and neuroplasticity related genes to elevated CO2 levels in the brain of three teleost species. Dryad Digital Repository. https://doi.org/10.5061/dryad.pn397
Cite | Share
Download the data package citation in the following formats:
   RIS (compatible with EndNote, Reference Manager, ProCite, RefWorks)
   BibTex (compatible with BibDesk, LaTeX)

Search for data

Be part of Dryad

We encourage organizations to: