Data from: Cyanobacteria maintain constant protein concentration despite genome copy-number variation

Zheng, Xiao-yu, Harvard University

O'Shea, Erin K., Harvard University

Published Mar 31, 2018 on Dryad. https://doi.org/10.5061/dryad.ps319

Cite this dataset

Zheng, Xiao-yu; O'Shea, Erin K. (2018). Data from: Cyanobacteria maintain constant protein concentration despite genome copy-number variation [Dataset]. Dryad. https://doi.org/10.5061/dryad.ps319

Abstract

The cyanobacterium Synechococcus elongatus PCC 7942 has multiple copies of its single chromosome, and the copy number varies in individual cells, providing an ideal system to study the effect of genome copy-number variation on cell size and gene expression. Using single-cell fluorescence imaging, we found that protein concentration remained constant across individual cells regardless of genome copy number. Cell volume and the total protein amount from a single gene were both positively, linearly correlated with genome copy number, suggesting that changes in cell volume play an important role in buffering genome copy-number variance. This study provides a quantitative examination of gene expression regulation in cells with variable genome copies and sheds light on the compensation mechanisms for variance in genome copy number.

Usage notes

Read_me

BbbJ23119_30uE

Raw images for the strain BbbJ23119::EYFP grown in default condition. Corresponding to data presented in Figure 1, Figure 2, Figure 1S and Figure 4S. Corresponding to 'BbbJ23119_30uE_r1' data in supplemental data and Table S1.

BbbJ23119_Dark

Raw images for strain BbbJ23119::EYFP after 24 h darkness. Corresponding to data presented in Figure 3, 'BbbJ23119_Dark' in supplemental data S1 and that in Table S1.

BbbJ23119_Black.zip

BbbJ23119_High

Raw images for strain BbbJ23119::EYFP strain grown under high light intensity (Figure S3). Corresponding to ‘BbbJ23119_High_r1’ in supplemental data S1 and Table S1.

BbbJ23119_Low

Raw images for strain BbbJ23119::EYFP strain grown under low light intensity (Figure 3). Corresponding to ‘BbbJ23119_Low_r1’ in supplemental data S1 and Table S1.

BbbJ23119_minD

Raw images for minD mutant strain with BbbJ23119::EYFP strain grown under default condition (Figure 4 and Figure S4). Corresponding to ‘BbbJ23119_minD_r1’ in supplemental data S1 and Table S1.

pkaiBC_ZT6

Raw images for strain pkaiBC::EYFP strain at ZT6, trough phase (Figure 1E and Figure S2). Corresponding to 'pkaiBC_ZT6_r1’ in supplemental data S1 and Table S1.

pKaiBC_ZT6.zip

pkaiBC_ZT18

Raw images for strain pkaiBC::EYFP strain at ZT18, peak phase (Figure 1E and Figure S2). Corresponding to 'pkaiBC_ZT18_r1’ in supplemental data S1 and Table S1.