Data from: A RhoG-mediated signaling pathway that modulates invadopodia dynamics in breast cancer cells

Goicoechea SM, Zinn A, Awadia SS, Snyder K, Garcia-Mata R

Date Published: January 17, 2017

DOI: http://dx.doi.org/10.5061/dryad.q1j50

 

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Title Supplementary Movie 1. Invadopodia formation in CTRL SUM159 cells in response to PDBu.
Downloaded 15 times
Description SUM159 cells expressing a CTRL shRNA were infected with mCherry-cortactin adenovirus and plated on glass-bottom coverslips 24 h after infection. Cells were imaged at 37°C in a CO2 and humidity controlled environment. At indicated times cells were stimulated to form invadopodia with PDBu. Images were captured at 15 sec intervals for the duration of the movie.
Download Supp Movie 1-non targeting 360x480_15fps.mov (1.096 Mb)
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Title Supplementary Movie 2. Invadopodia formation in Rac1 KO cells.
Downloaded 12 times
Description Rac1 KO SUM159 cells were infected with adenovirus encoding mCherry-cortactin as in Supp Movie 1. At indicated times cells were stimulated to form invadopodia with PDBu. Images were captured at 15 sec intervals for the duration of the movie.
Download Supp Movie 2-Rac KO-360x480.mov (1.895 Mb)
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Title Supplementary Movie 3. Invadopodia formation in Rac1 KO/rescue cells.
Downloaded 9 times
Description Rac1 KO SUM159 cells were co-infected with adenovirus encoding mCherry-cortactin and mycRac1. At indicated times cells were stimulated to form invadopodia with PDBu. Images were captured at 15 sec intervals for the duration of the movie.
Download Supp Movie 3-Rac KO- Rescue_360x480.mov (2.278 Mb)
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Title Supplementary Movie 4. Invadopodia in RhoG KD SUM159 cells.
Downloaded 9 times
Description SUM159 cells stably expressing a RhoG targeting shRNA were infected with mCherry-cortactin adenovirus as in Supp Movie 1. At indicated times cells were stimulated to form invadopodia with PDBu. Images were captured at 15 sec intervals for the duration of the movie.
Download Supp Movie 4_RhoG KD_480x360 37min.mov (2.373 Mb)
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Title Supplementary Movie 5. Invadopodia formation in SGEF KD cells.
Downloaded 10 times
Description SUM159 cells stably expressing SGEF targeting shRNA were infected with mCherry-cortactin adenovirus as in Supp Movie 1. At indicated times cells were stimulated to form invadopodia with PDBu. Images were captured at 15 sec intervals for the duration of the movie.
Download Supp Movie 5 -SGEF-KD-360x480.mov (4.777 Mb)
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Title Supplementary Movie 6. Invadopodia formation in RhoG KD/rescue cells.
Downloaded 5 times
Description RhoG KD SUM159 cells were co-infected with adenoviruses encoding mCherry-cortactin and shRNA resistant mycRhoG. At indicated times cells were stimulated to form invadopodia with PDBu. Images were captured at 15 sec intervals for the duration of the movie.
Download Supp Movie 6-RhoG-rescue_480x360.mov (1.749 Mb)
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Title Supplementary Movie 7. Invadopodia formation in SGEF KD/rescue cells.
Downloaded 7 times
Description SGEF KD SUM159 cells were co-infected with adenovirus encoding mCherry-cortactin and shRNA resistant mycSGEF. At indicated times cells were stimulated to form invadopodia with PDBu. Images were captured at 15 sec intervals for the duration of the movie.
Download Supp Movie 7_SGEF-rescue 480x360.mov (3.071 Mb)
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When using this data, please cite the original publication:

Goicoechea SM, Zinn A, Awadia SS, Snyder K, Garcia-Mata R (2017) A RhoG-mediated signaling pathway that modulates invadopodia dynamics in breast cancer cells. Journal of Cell Science 130(6): 1064-1077. http://dx.doi.org/10.1242/jcs.195552

Additionally, please cite the Dryad data package:

Goicoechea SM, Zinn A, Awadia SS, Snyder K, Garcia-Mata R (2017) Data from: A RhoG-mediated signaling pathway that modulates invadopodia dynamics in breast cancer cells. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.q1j50
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