Data from: Nuclear microenvironments modulate transcription from low-affinity enhancers

Tsai A, Muthusamy AK, Alves MRP, Lavis LD, Singer RH, Stern DL, Crocker J

Date Published: November 15, 2017

DOI: https://doi.org/10.5061/dryad.q96g6.2

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Title Airyscan Alexa568-Ubx Alexa488-svb DAPI Embryo 01_01
Downloaded 1 time
Description Embryo 01 imaging area 01. Airyscan imaging in the a1 segment of a fixed stage 15 Drosophila melanogaster embryo. Channel 1: Ubx labeled with Alexa 568 using IF. Channel 2: Transcription sites of svb labeled with Alexa 488 using IF against DIG-RNA probes targeting the first intronic region of svb. Channel 3: DAPI.
Download Alexa568 Ubx Alexa488 svb DAPI Embryo 01_01.tif (180.1 Mb)
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Title Airyscan Alexa568-Ubx Alexa488-svb DAPI Embryo 02_01
Description Embryo 02 imaging area 01. Airyscan imaging in the a1 segment of a fixed stage 15 Drosophila melanogaster embryo. Channel 1: Ubx labeled with Alexa 568 using IF. Channel 2: Transcription sites of svb labeled with Alexa 488 using IF against DIG-RNA probes targeting the first intronic region of svb. Channel 3: DAPI.
Download Alexa568 Ubx Alexa488 svb DAPI Embryo 02_01.tif (301.8 Mb)
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Title Airyscan Alexa568-Ubx Alexa488-svb DAPI Embryo 02_02
Description Embryo 02 imaging area 02. Airyscan imaging in the a1 segment of a fixed stage 15 Drosophila melanogaster embryo. Channel 1: Ubx labeled with Alexa 568 using IF. Channel 2: Transcription sites of svb labeled with Alexa 488 using IF against DIG-RNA probes targeting the first intronic region of svb. Channel 3: DAPI.
Download Alexa568 Ubx Alexa488 svb DAPI Embryo 02_02.tif (452.7 Mb)
Details View File Details
Title Airyscan Alexa568-Ubx Alexa488-svb DAPI Embryo 03_01
Description Embryo 03 imaging area 01. Airyscan imaging in the a1 segment of a fixed stage 15 Drosophila melanogaster embryo. Channel 1: Ubx labeled with Alexa 568 using IF. Channel 2: Transcription sites of svb labeled with Alexa 488 using IF against DIG-RNA probes targeting the first intronic region of svb. Channel 3: DAPI.
Download Alexa568 Ubx Alexa488 svb DAPI Embryo 03_01.tif (493.9 Mb)
Details View File Details
Title Airyscan Alexa568-Ubx Alexa488-svb DAPI Embryo 03_02
Description Embryo 03 imaging area 02. Airyscan imaging in the a1 segment of a fixed stage 15 Drosophila melanogaster embryo. Channel 1: Ubx labeled with Alexa 568 using IF. Channel 2: Transcription sites of svb labeled with Alexa 488 using IF against DIG-RNA probes targeting the first intronic region of svb. Channel 3: DAPI.
Download Alexa568 Ubx Alexa488 svb DAPI Embryo 03_02.tif (480.2 Mb)
Details View File Details
Title Airyscan Alexa568-Ubx Alexa488-svb DAPI Embryo 04_01
Description Embryo 04 imaging area 01. Airyscan imaging in the a1 segment of a fixed stage 15 Drosophila melanogaster embryo. Channel 1: Ubx labeled with Alexa 568 using IF. Channel 2: Transcription sites of svb labeled with Alexa 488 using IF against DIG-RNA probes targeting the first intronic region of svb. Channel 3: DAPI.
Download Alexa568 Ubx Alexa488 svb DAPI Embryo 04_01.tif (507.6 Mb)
Details View File Details
Title Airyscan Alexa568-Ubx Alexa488-svb DAPI Embryo 04_02
Description Embryo 04 imaging area 02. Airyscan imaging in the a1 segment of a fixed stage 15 Drosophila melanogaster embryo. Channel 1: Ubx labeled with Alexa 568 using IF. Channel 2: Transcription sites of svb labeled with Alexa 488 using IF against DIG-RNA probes targeting the first intronic region of svb. Channel 3: DAPI.
Download Alexa568 Ubx Alexa488 svb DAPI Embryo 04_02.tif (208.3 Mb)
Details View File Details
Title Live widefield JF635-HaloTag-NLS stage 5 embryo
Description Wide field imaging in the anterior region of a live stage 5 Drosophila melanogaster embryo expressing HaloTag-NLS. The HaloTag-NLS protein is unstable and degraded after translation. The embryo is injected with JF635 linked to a HaloTag ligand 2 hours after egg-laying. The embryo is epi-illuminated with a 633 nm laser and videos are captured with an exposure time of 100 ms.
Download HaloTag-NLS.tif (262.3 Mb)
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Title Live widefield JF635-HaloTag-Ubx(R3A N51A) stage 5 embryo
Description Wide field imaging in the anterior region of a live stage 5 Drosophila melanogaster embryo expressing HaloTag-Ubx(R3A N51A). This Ubx is DNA-binding deficient. The embryo is injected with JF635 linked to a HaloTag ligand 2 hours after egg-laying. The embryo is epi-illuminated with a 633 nm laser and videos are captured with an exposure time of 100 ms.
Download HaloTag-binding deficient Ubx.tif (524.7 Mb)
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Title Live widefield JF635-HaloTag-Ubx late stage 6 embryo
Description Wide field imaging in the anterior region of a live late stage 6 Drosophila melanogaster embryo expressing HaloTag-Ubx. The embryo is injected with JF635 linked to a HaloTag ligand 2 hours after egg-laying. The embryo is epi-illuminated with a 633 nm laser and videos are captured with an exposure time of 100 ms.
Download HaloTag-Ubx old embryo.tif (529.4 Mb)
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Title Live widefield JF635-HaloTag-Ubx stage 5 embryo
Description Wide field imaging in the anterior region of a live stage 5 Drosophila melanogaster embryo expressing HaloTag-Ubx. This Ubx is the wildtype protein. The embryo is injected with JF635 linked to a HaloTag ligand 2 hours after egg-laying. The embryo is epi-illuminated with a 633 nm laser and videos are captured with an exposure time of 100 ms.
Download HaloTag-Ubx.tif (1.049 Gb)
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When using this data, please cite the original publication:

Tsai A, Muthusamy AK, Alves MR, Lavis LD, Singer RH, Stern DL, Crocker J (2017) Nuclear microenvironments modulate transcription from low-affinity enhancers. eLife 6: e28975. https://doi.org/10.7554/elife.28975

Additionally, please cite the Dryad data package:

Tsai A, Muthusamy AK, Alves MRP, Lavis LD, Singer RH, Stern DL, Crocker J (2017) Data from: Nuclear microenvironments modulate transcription from low-affinity enhancers. Dryad Digital Repository. https://doi.org/10.5061/dryad.q96g6.2
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