Data from: Loss of growth homeostasis by genetic decoupling of cell division from biomass growth: implication for size control mechanisms

Schmidt-Glenewinkel H, Barkai N

Date Published: July 1, 2015

DOI: http://dx.doi.org/10.5061/dryad.r4n35

 

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Title wildtype cells grown in 0%, 0.01%, 0.1% or 0.2% glucose
Downloaded 16 times
Description This .nd2 file can be viewed with the Nikon Viewer, available at http://www.nikoninstruments.com/Products/Software/NIS-Elements-Advanced-Research/NIS-Elements-Viewer. The file contains 80 fields of view (FOVs), images were taken every 10 minutes. The data corresponds to figures 1A, 1B and 1C. Wildtype cells were grown in SC media supplemented with either no glucose (FOV 1 to 20, up to timepoint 144), 0.01% glucose (FOV 21 to 40), 0.1% glucose (FOV 41 to 60) or 0.2% glucose (FOV 61 to 80).
Download 121205_WT.nd2 (34.46 Gb)
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Title wildtype cells grown 2% glucose
Downloaded 18 times
Description Wildtype cells grown in SC with 2% glucose. Fields of view 27 to 41. Images were taken every 10 minutes. Corresponds to figure 1B, 1C.
Download ma10_WT_KD_1condition001.nd2 (21.68 Gb)
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Title Transporterless cells with sensors in 0% glucose
Downloaded 22 times
Description Transporterless cells with sensors were grown in 0% glucose. Fields of view 1 to 20. Images were taken every 10 minutes.
Download 121017_sys_D1_Dox0.nd2 (20.00 Gb)
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Title Transporterless cells without sensors in 0% or 2% glucose
Downloaded 12 times
Description Transporterless cells without sensors were grown in 0% (Fields of view 1 to 20) or 2% (Fields of view 61 to 80) glucose. Corresponds to figures 1H (2%) and 1I (0%) Images were taken every 10 minutes.
Download 121123_Y1.nd2 (30.42 Gb)
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Title single-HXT2 with sensors in 0.1% glucose, 125ng/ml DOX
Downloaded 7 times
Description single-HXT2 with sensors were grown in 0.1% glucose, with 125ng/ml DOX. Fields of view 41 to 60.
Download 121115_D2_Dox125ng002.nd2 (30.42 Gb)
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Title single-HXT2 with sensors in 0.1% glucose, 250ng/ml DOX
Downloaded 10 times
Description single-HXT2 with sensors were grown in 0.1% glucose, with 250ng/ml DOX. Fields of view 41 to 60.
Download 121111_D2_Dox250ng.nd2 (30.42 Gb)
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Title single-HXT2 with sensors in 0.1% glucose, 1250ng/ml DOX
Downloaded 8 times
Description single-HXT2 with sensors were grown in 0.1% glucose, with 1250ng/ml DOX. Field of view 41 to 60.
Download 121114_D2_Dox1p25ug001.nd2 (24.37 Gb)
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Title single-HXT2 with sensors in 0.1% glucose, 2500ng/ml DOX
Downloaded 13 times
Description single-HXT2 with sensors were grown in 0.1% glucose, with 2500ng/ml DOX. Field of view 41 to 60.
Download 121113_D2_Dox2p5ug.nd2 (18.15 Gb)
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Title single-HXT2 without sensors in 0.1% glucose, no DOX
Downloaded 10 times
Description single-HXT2 without sensors were grown in 0.1% glucose, with DOX. Fields of view 1 to 20
Download 130116_Y4noDox.nd2 (30.42 Gb)
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Title single-HXT2 without sensors in 0.1% glucose, 2500 ng/ml DOX AND single-HXT4 without sensors in 0.1% or 2% glucose 2500ng/ml DOX
Downloaded 9 times
Description Single-HXT2 without sensors were grown in 0.1% glucose, with 2500ng/ml DOX. Field of view 41 to 60. Single-HXT4 without sensors were grown in 0.1% (Fields of view 41 to 60) or 2% (Fields of view 61 to 80) glucose, with 2500ng/ml DOX.
Download 130121_Y4_2p5Dox001.nd2 (30.42 Gb)
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When using this data, please cite the original publication:

Schmidt-Glenewinkel H, Barkai N (2014) Loss of growth homeostasis by genetic decoupling of cell division from biomass growth: implication for size control mechanisms. Molecular Systems Biology 10: 769. http://dx.doi.org/10.15252/msb.20145513

Additionally, please cite the Dryad data package:

Schmidt-Glenewinkel H, Barkai N (2014) Data from: Loss of growth homeostasis by genetic decoupling of cell division from biomass growth: implication for size control mechanisms. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.r4n35
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