Data from: Evaluation of the impact of RNA preservation methods of spiders for de novo transcriptome assembly

Kono N, Nakamura H, Ito Y, Tomita M, Arakawa K

Date Published: November 9, 2015

DOI: http://dx.doi.org/10.5061/dryad.r94k7

 

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Title Fasta file of COI sequence from all 24 samples and one reference assembly
Downloaded 14 times
Description This fast file lists all COI sequences from all 24 samples and one reference assembly used in this work to confirm the species to be Parasteatoda tepidariorum.
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Title Fasta file of 18S rDNA sequences from all 24 samples and one reference assembly used in this work
Downloaded 5 times
Description This fast file lists all COI sequences from all 24 samples and one reference assembly used in this work to confirm the species to be Parasteatoda tepidariorum.
Download 18S_all_comp.fasta (23.67 Kb)
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Title Input and Output Files for DEG analysis using edgeR
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Description Input count data (*_input.txt files, calculated by kallisto software) and output DEG analysis data (*_output.txt files, calculated by edgeR tool). 5M, 20M, or 30M indicates the number of subsampled reads used in the analysis. 1. Files with NO80_p[1-7]_[5|20|30]M_[input/output].txt or NO80_s[1-6]_[5|20|30]M_[input/output].txt These files were for the calculation of Fig. 2b, where NO80_p1_* NO80 vs RIN1.0, 2.2 NO80_p2_* NO80 vs RIN2.2, 3.8 NO80_p3_* NO80 vs RIN3.8, 5.3 NO80_p4_* NO80 vs RIN5.3, 6.3 NO80_p5_* NO80 vs RIN6.3, 7.1 NO80_p6_* NO80 vs RIN7.1, 8.0 NO80_p7_* NO80 vs RIN8.0, 9.0 NO80_s1_* NO80 vs RIN1.0, 2.2, 3.8 NO80_s2_* NO80 vs RIN2.2, 3.8, 5.3 NO80_s3_* NO80 vs RIN3.8, 5.3, 6.3 NO80_s4_* NO80 vs RIN5.3, 6.3, 7.1 NO80_s5_* NO80 vs RIN6.3, 7.1, 8.0 NO80_s6_* NO80 vs RIN7.1, 8.0, 9.0 2. Other files were used for the calculation of Table 3, where AE: Directly submerged in Ethanol AR: Directly submerged in RNAlater CE: Crushed and submerged in Ethanol CR: Crushed and submerged in RNAlater CT: Crushed and submerged in TRIzol NO: Flash-frozen in liquid nitrogen
Download edgeR_OUT_IN_files.zip (349.3 Mb)
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When using this data, please cite the original publication:

Kono N, Nakamura H, Ito Y, Tomita M, Arakawa K (2015) Evaluation of the impact of RNA preservation methods of spiders for de novo transcriptome assembly. Molecular Ecology Resources 16(3): 662-672. http://dx.doi.org/10.1111/1755-0998.12485

Additionally, please cite the Dryad data package:

Kono N, Nakamura H, Ito Y, Tomita M, Arakawa K (2015) Data from: Evaluation of the impact of RNA preservation methods of spiders for de novo transcriptome assembly. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.r94k7
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