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dc.contributor.author Morgan-Richards, Mary
dc.contributor.author Bulgarella, Mariana
dc.contributor.author Sivyer, Louisa
dc.contributor.author Dowle, Edwina J.
dc.contributor.author Hale, Marie
dc.contributor.author van Heugten, Rachael
dc.contributor.author McKean, Natasha E.
dc.contributor.author Trewick, Steven A.
dc.coverage.spatial New Zealand
dc.coverage.temporal recent
dc.date.accessioned 2017-10-27T13:38:04Z
dc.date.available 2017-10-27T13:38:04Z
dc.date.issued 2017-11-29
dc.identifier doi:10.5061/dryad.rg15p
dc.identifier.citation Morgan-Richards M, Bulgarella M, Sivyer L, Dowle EJ, Hale M, McKean NE, Trewick SA (2017) Explaining large mitochondrial sequence differences within a population sample. Royal Society Open Science 4(11): 170730.
dc.identifier.issn 2054-5703
dc.identifier.uri http://hdl.handle.net/10255/dryad.149672
dc.description Mitochondrial DNA sequence is frequently used to infer species' boundaries, as divergence is relatively rapid when populations are reproductively isolated. However, the shared history of a non-recombining gene naturally leads to correlation of pairwise differences, resulting in mtDNA clusters that might be mistaken for evidence of multiple species. There are four distinct processes that can explain high levels of mtDNA sequence difference within a single sample. Here, we examine one case in detail as an exemplar to distinguish among competing hypotheses. Within our sample of tree wētā (Hemideina crassidens; Orthoptera), we found multiple mtDNA haplotypes for a protein-coding region (cytb/ND1) that differed by a maximum of 7.9%. From sequencing the whole mitochondrial genome of two representative individuals, we found evidence of constraining selection. Heterozygotes were as common as expected under random mating at five nuclear loci. Morphological traits and nuclear markers did not resolve the mtDNA groupings of individuals. We concluded that the large differences found among our sample of mtDNA sequences were simply owing to a large population size over an extended period of time allowing an equilibrium between mutation and drift to retain a great deal of genetic diversity within a single species.
dc.relation.haspart doi:10.5061/dryad.rg15p/1
dc.relation.haspart doi:10.5061/dryad.rg15p/2
dc.relation.haspart doi:10.5061/dryad.rg15p/3
dc.relation.isreferencedby doi:10.1098/rsos.170730
dc.subject Constraining selection
dc.subject DNA barcoding
dc.subject genetic drift
dc.subject mtDNA divergence
dc.subject population size
dc.title Data from: Explaining large mitochondrial sequence differences within a population sample
dc.type Article
dwc.ScientificName Hemideina crassidens
dc.contributor.correspondingAuthor Morgan-Richards, Mary
prism.publicationName Royal Society Open Science

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Title Cytb-ND_rangi_29specimens
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Description Short (519 bp) mtDNA sequences from a population sample of the tree weta Hemideina crassidens.
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Title Phenotype and genotypes for pop sample_nuclear markers_table
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Description Excel table with two morphological traits and genotypes at five nuclear loci provided for 29 tree weta (Hemideina crassidens) collected from the same location
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Title Hemideina_crassidens_mtDNAgenomes.fasta
Downloaded 1 time
Description Whole mitochondrial genome sequences from two tree weta (Hemideina crassidens) representing two clades observed within a single population sample
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