Data from: Rust disease of eucalypts, caused by Puccinia psidii, did not originate via host jump from guava in Brazil

 

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Title Geographic coordinates and allele scores of Puccinia psidii samples from Brazil and Uruguay
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Description Data include geographic coordinates of single uredinial pustules of Puccinia psidii ca. 6 mm diameter collected between March 2008 and August 2009 from 148 individual plants representing seven myrtaceous taxa (eucalypts, guava, rose apple, Brazilian guava, Java plum, jabuticaba, and pitanga) in nine Brazilian states and one location in Uruguay. Samples were genotyped at 10 microsatellite loci (PpSSR012, PpSSR014, PpSSR018, PpSSR022, PpSSR087, PpSSR102, PpSSR146, PpSSR161, PpSSR178, and PpSSR195) using genomic DNA extracted directly from each pustule following a modified CTAB-based protocol. Fragment analysis was via capillary electrophoresis using an ABI 3700 DNA automated sequencer. Positive and negative controls were included for each locus scored and scoring was repeated for representative alleles for each locus to ensure the accuracy of genotyping. Allele sizes were estimated using marker standards (ROX Geneflo 625, CHIMERx, Milwaukee, WI, USA) and scored using ABI PeakScanner Analysis Software v1.0.
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When using this data, please cite the original publication:

Graça RN, Ross-Davis AL, Klopfenstein NB, Kim M, Peever TL, Cannon PG, Aun CP, Mizubuti ESG, Alfenas AC (2013) Rust disease of eucalypts, caused by Puccinia psidii, did not originate via host jump from guava in Brazil. Molecular Ecology 22(24): 6033–6047. http://dx.doi.org/10.1111/mec.12545

Additionally, please cite the Dryad data package:

Graça RN, Ross-Davis AL, Klopfenstein NB, Kim M, Peever TL, Cannon PG, Aun CP, Mizubuti ESG, Alfenas AC (2013) Data from: Rust disease of eucalypts, caused by Puccinia psidii, did not originate via host jump from guava in Brazil. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.rk7n8
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