Data from: Rapid diversification of sperm precedence traits and processes among three sibling Drosophila species

Manier MK, Belote JM, Berben KS, Lüpold S, Ala-Honkola O, Collins WF, Pitnick S

Date Published: March 15, 2013

DOI: http://dx.doi.org/10.5061/dryad.s1092

 

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Title Virgin.sim
Downloaded 7 times
Description Data on sperm transfer and storage after first mating for D. simulans. Variables: Treat – treatment, transfer or storage; Male – transgenic line by sperm label, GFP or RFP; Cop – copulation duration in minutes; Bursa – number of sperm in the bursa; Prox – number of sperm in the proximal half of the seminal receptacle (SR); Dist – number of sperm in the distal half of the SR; Caps – number of sperm in the paired spermathecal caps combined; Ducts – number of sperm in the paired spermathecal ducts combined
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Title Virgin.maur
Downloaded 8 times
Description Data on sperm transfer and storage after first mating for D. mauritiana. Variables: Treat – treatment, transfer or storage; Male – transgenic line by sperm label, GFP or RFP; Cop – copulation duration in minutes; Bursa – number of sperm in the bursa; Prox – number of sperm in the proximal half of the seminal receptacle (SR); Dist – number of sperm in the distal half of the SR; Caps – number of sperm in the paired spermathecal caps combined; Ducts – number of sperm in the paired spermathecal ducts combined
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Title Remate.sim
Downloaded 6 times
Description Sperm numbers in space and time after second mating for D. simulans. Variables: MinASM – time after the start of mating at which female was frozen; FirstM – transgenic line of first male by sperm label, GFP or RFP. Second male is automatically the reciprocal label; Remate – day after first mating (day 0) on which female remated; Cop1 – duration in minutes of the first copulation; Cop2 – duration in minutes of the second copulation; PriorProg – number of progeny produced by female before remating; Bursa1 – number of first-male sperm in the bursa; Bursa2 – number of second-male sperm in the bursa; Prox1 – number of first-male sperm in the proximal half of the SR; Prox2 – number of second-male sperm in the proximal half of the SR; Dist1 – number of first-male sperm in the distal half of the SR; Dist1 – number of first-male sperm in the distal half of the SR; Dist2 – number of second-male sperm in the distal half of the SR; Caps1 – number of first-male sperm in the two spermathecal caps combined; Caps2 – number of second-male sperm in the two spermathecal caps combined; Ducts1 – number of first-male sperm in the two spermathecal ducts combined; Ducts2 – number of second-male sperm in the two spermathecal ducts combined
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Title Remate.maur
Downloaded 6 times
Description Sperm numbers in space and time after second mating for D. mauritiana. Variables: MinASM – time after the start of mating at which female was frozen; FirstM – transgenic line of first male by sperm label, GFP or RFP. Second male is automatically the reciprocal label; Remate – day after first mating (day 0) on which female remated; Cop1 – duration in minutes of the first copulation; Cop2 – duration in minutes of the second copulation; PriorProg – number of progeny produced by female before remating; Bursa1 – number of first-male sperm in the bursa; Bursa2 – number of second-male sperm in the bursa; Prox1 – number of first-male sperm in the proximal half of the SR; Prox2 – number of second-male sperm in the proximal half of the SR; Dist1 – number of first-male sperm in the distal half of the SR; Dist1 – number of first-male sperm in the distal half of the SR; Dist2 – number of second-male sperm in the distal half of the SR; Caps1 – number of first-male sperm in the two spermathecal caps combined; Caps2 – number of second-male sperm in the two spermathecal caps combined; Ducts1 – number of first-male sperm in the two spermathecal ducts combined; Ducts2 – number of second-male sperm in the two spermathecal ducts combined
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Title Remate.mel
Downloaded 5 times
Description Sperm numbers in space and time after second mating for D. melanogaster. Variables: Block - different experimental blocks, A and B; MinASM – time after the start of mating at which female was frozen; FirstM – transgenic line of first male by sperm label, GFP or RFP. Second male is automatically the reciprocal label; Remate – day after first mating (day 0) on which female remated; Cop2 – duration in minutes of the second copulation; Bursa1 – number of first-male sperm in the bursa; Bursa2 – number of second-male sperm in the bursa; Prox1 – number of first-male sperm in the proximal half of the SR; Prox2 – number of second-male sperm in the proximal half of the SR; Dist1 – number of first-male sperm in the distal half of the SR; Dist1 – number of first-male sperm in the distal half of the SR; Dist2 – number of second-male sperm in the distal half of the SR; Caps1 – number of first-male sperm in the two spermathecal caps combined; Caps2 – number of second-male sperm in the two spermathecal caps combined; Ducts1 – number of first-male sperm in the two spermathecal ducts combined; Ducts2 – number of second-male sperm in the two spermathecal ducts combined
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Title Ejection.sim
Downloaded 4 times
Description Timing and number of sperm ejected after mating for D. simulans. Variables: Block – different experimental blocks, A and B; FirstM – transgenic line of first male by sperm label, GFP or RFP. Second male is automatically the reciprocal label; Mates – number of times each female mated; Remate – day after first mating (day 0) on which female remated; Cop1 – duration in minutes of the first copulation; Cop2 – duration in minutes of the second copulation; EjTime1 –Ejection time after first mating, in minutes after the start of the mating; EjTime2 – Ejection time after second mating, in minutes after the start of mating; PriorProg – number of progeny produced by female before remating; Eject1 – number of first-male sperm in ejection after first mating; EjMale1 – number of first-male sperm in ejection after second mating; EjMale2 – number of second-male sperm in ejection after second mating; Bursa1 – number of first-male sperm in the bursa; Bursa2 – number of second-male sperm in the bursa; Prox1 – number of first-male sperm in the proximal half of the SR; Prox2 – number of second-male sperm in the proximal half of the SR; Dist1 – number of first-male sperm in the distal half of the SR; Dist1 – number of first-male sperm in the distal half of the SR; Dist2 – number of second-male sperm in the distal half of the SR; Caps1 – number of first-male sperm in the two spermathecal caps combined; Caps2 – number of second-male sperm in the two spermathecal caps combined; Ducts1 – number of first-male sperm in the two spermathecal ducts combined; Ducts2 – number of second-male sperm in the two spermathecal ducts combined
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Title Ejection.maur
Downloaded 5 times
Description Timing and number of sperm ejected after mating for D. mauritiana. Variables: Block – different experimental blocks, A and B; FirstM – transgenic line of first male by sperm label, GFP or RFP. Second male is automatically the reciprocal label; Mates – number of times each female mated; Remate – day after first mating (day 0) on which female remated; Cop1 – duration in minutes of the first copulation; Cop2 – duration in minutes of the second copulation; EjTime1 –Ejection time after first mating, in minutes after the start of the mating; EjTime2 – Ejection time after second mating, in minutes after the start of mating; PriorProg – number of progeny produced by female before remating; Eject1 – number of first-male sperm in ejection after first mating; EjMale1 – number of first-male sperm in ejection after second mating; EjMale2 – number of second-male sperm in ejection after second mating; Bursa1 – number of first-male sperm in the bursa; Bursa2 – number of second-male sperm in the bursa; Prox1 – number of first-male sperm in the proximal half of the SR; Prox2 – number of second-male sperm in the proximal half of the SR; Dist1 – number of first-male sperm in the distal half of the SR; Dist1 – number of first-male sperm in the distal half of the SR; Dist2 – number of second-male sperm in the distal half of the SR; Caps1 – number of first-male sperm in the two spermathecal caps combined; Caps2 – number of second-male sperm in the two spermathecal caps combined; Ducts1 – number of first-male sperm in the two spermathecal ducts combined; Ducts2 – number of second-male sperm in the two spermathecal ducts combined
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Title Velocity.sim
Downloaded 7 times
Description Sperm velocity and sperm numbers in the SR for D. simulans. Variables: Fem – identity assigned to individual female; Movie – name of movie file from which data were collected; Treat – treatment; A = virgin mating, B = remating, C = no remate; Region – region of the female reproductive tract, bursa or SR; Male – first- or second-male sperm; Velmps – instantaneous linear velocity in microns per second, averaged for each sperm and for all sperm recorded in a movie; N – number of sperm tracked per movie; SR1 – number of first-male sperm in the SR; SR2 – number of second-male sperm in the SR
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Title Velocity.maur
Downloaded 6 times
Description Sperm velocity and sperm numbers in the SR for D. mauritiana. Variables: Fem – identity assigned to individual female; Movie – name of movie file from which data were collected; Treat – treatment; A = virgin mating, B = remating, C = no remate; Region – region of the female reproductive tract, bursa or SR; Male – first- or second-male sperm; Velmps – instantaneous linear velocity in microns per second, averaged for each sperm and for all sperm recorded in a movie; N – number of sperm tracked per movie; SR1 – number of first-male sperm in the SR; SR2 – number of second-male sperm in the SR
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Title Velocity.mel
Downloaded 8 times
Description Sperm velocity and sperm numbers in the SR for D. melanogaster. Variables: Fem – identity assigned to individual female; Movie – name of movie file from which data were collected; Treat – treatment (A = virgin mating, B = remating, C = no remate); Region – region of the female reproductive tract, bursa or SR; Male – first- or second-male sperm; Velmps – instantaneous linear velocity in microns per second, averaged for each sperm and for all sperm recorded in a movie; N – number of sperm tracked per movie; SR1 – number of first-male sperm in the SR; SR2 – number of second-male sperm in the SR
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Title P2.sim
Downloaded 3 times
Description Paternity and sperm count data for D. simulans. Variables: Treat – time after mating for which paternity and sperm numbers in the female reproductive tract were quantified; FirstM – transgenic line of first male by sperm label, GFP or RFP. Second male is automatically the reciprocal label; Remate – day after first mating (day 0) on which female remated; Cop1 – duration in minutes of the first copulation; Cop2 – duration in minutes of the second copulation; PriorProg – number of progeny produced by female before remating; P2.24 – proportion of progeny sired by the second male (P2) for the first 24 hrs after mating; P2.48 – P2 for the first 48 hrs after mating; P2.72 - P2 for the first 72 hrs after mating ; P2 – P2 over entire time period; Bursa1 – number of first-male sperm in the bursa; Bursa2 – number of second-male sperm in the bursa; Prox1 – number of first-male sperm in the proximal half of the SR; Prox2 – number of second-male sperm in the proximal half of the SR; Dist1 – number of first-male sperm in the distal half of the SR; Dist1 – number of first-male sperm in the distal half of the SR; Dist2 – number of second-male sperm in the distal half of the SR; Caps1 – number of first-male sperm in the two spermathecal caps combined; Caps2 – number of second-male sperm in the two spermathecal caps combined; Ducts1 – number of first-male sperm in the two spermathecal ducts combined; Ducts2 – number of second-male sperm in the two spermathecal ducts combined; SR1 – number of first-male sperm in the entire SR; SR2 – number of second-male sperm in the entire SR; Sp1 – number of first-male sperm in both spermathecae combined; Sp2 – number of second-male sperm in both spermathecae combined
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Title P2.maur
Downloaded 4 times
Description Paternity and sperm count data for D. mauritiana. Variables: Block – for maur only, different experimental blocks, A and B; Treat – time after mating for which paternity and sperm numbers in the female reproductive tract were quantified; FirstM – transgenic line of first male by sperm label, GFP or RFP. Second male is automatically the reciprocal label; Remate – day after first mating (day 0) on which female remated; Cop1 – duration in minutes of the first copulation; Cop2 – duration in minutes of the second copulation; PriorProg – number of progeny produced by female before remating; P2.24 – proportion of progeny sired by the second male (P2) for the first 24 hrs after mating; P2.48 – P2 for the first 48 hrs after mating; P2.72 - P2 for the first 72 hrs after mating; P2 – P2 over entire time period; Bursa1 – number of first-male sperm in the bursa; Bursa2 – number of second-male sperm in the bursa; Prox1 – number of first-male sperm in the proximal half of the SR; Prox2 – number of second-male sperm in the proximal half of the SR; Dist1 – number of first-male sperm in the distal half of the SR; Dist1 – number of first-male sperm in the distal half of the SR; Dist2 – number of second-male sperm in the distal half of the SR; Caps1 – number of first-male sperm in the two spermathecal caps combined; Caps2 – number of second-male sperm in the two spermathecal caps combined; Ducts1 – number of first-male sperm in the two spermathecal ducts combined; Ducts2 – number of second-male sperm in the two spermathecal ducts combined; SR1 – number of first-male sperm in the entire SR; SR2 – number of second-male sperm in the entire SR; Sp1 – number of first-male sperm in both spermathecae combined; Sp2 – number of second-male sperm in both spermathecae combined
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Title P2.mel
Downloaded 7 times
Description Paternity and sperm count data for D. melanogaster. Variables: Treat – time after mating for which paternity and sperm numbers in the female reproductive tract were quantified; P2 – P2 over entire time period; SR1 – number of first-male sperm in the entire SR; SR2 – number of second-male sperm in the entire SR; Sp1 – number of first-male sperm in both spermathecae combined; Sp2 – number of second-male sperm in both spermathecae combined
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Title Sperm.Length
Downloaded 3 times
Description Sperm lengths in mm for D. simulans and D. mauritiana. Variables: Species – sim or maur; Thorax – thorax length as a measure of body size, in an aritrary unit, to be entered as a covariate in analyses. Thorax length should be correlated with SR length but not sperm length.; Sperm1-5 – length in mm for each of 5 sperm measured per male. Average sperm lengths for each male are calculated from the four longest measurements.
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Title SR.Length
Downloaded 4 times
Description Seminal receptacle (SR) lengths in mm for D. simulans and D. mauritiana. Variables: Species – sim or maur; SR – SR length in mm; Thorax – thorax length as a measure of body size, in an aritrary unit, to be entered as a covariate in analyses. Thorax length should be correlated with SR length but not sperm length.; Sperm1-5 – length in mm for each of 5 sperm measured per male. Average sperm lengths for each male are calculated from the four longest measurements.
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When using this data, please cite the original publication:

Manier MK, Belote JM, Berben KS, Lüpold S, Ala-Honkola O, Collins WF, Pitnick S (2013) Rapid diversification of sperm precedence traits and processes among three sibling Drosophila species. Evolution 67(8): 2348-2362. http://dx.doi.org/10.1111/evo.12117

Additionally, please cite the Dryad data package:

Manier MK, Belote JM, Berben KS, Lüpold S, Ala-Honkola O, Collins WF, Pitnick S (2013) Data from: Rapid diversification of sperm precedence traits and processes among three sibling Drosophila species. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.s1092
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