Data from: An experimental study of the putative mechanism of a synthetic autonomous rotary DNA nanomotor

Dunn KE, Leake MC, Wollman AJM, Trefzer MA, Johnson S, Tyrrell AM

Date Published: February 24, 2017

DOI: http://dx.doi.org/10.5061/dryad.sj179

 

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Title Sequential strand displacement in a linear construct.
Downloaded 7 times
Description QCM-D data in .csv format. File contains overtone-normalized frequency changes for thirteenth overtone as a function of time, with the corresponding dissipation changes.
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Title Strand displacement in a folded nanostructure (triangle)
Downloaded 4 times
Description QCM-D data in .csv format. File contains overtone-normalized frequency changes for thirteenth overtone as a function of time, with the corresponding dissipation changes. Data is provided for three cases: 1. triangle folded on surface and supplied with unfolded reverse complement of triangle. 2. pre-folded triangle supplied with unfolded reverse complement of triangle. 3. pre-folded triangle supplied with folded reverse complement of triangle.
Download Triangle.csv (551.8 Kb)
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Title The rotary motor and its components.
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Description QCM-D data in .csv format. File contains overtone-normalized frequency changes for thirteenth overtone as a function of time, with the corresponding dissipation changes.Data corresponds to four experiments: 1. folded square A supplied with unfolded square B. 2. folded blocked square A supplied with unblocking strand and then unfolded square B. 3. folded square A supplied with folded square B. 4. full motor supplied sequentially with both unblocking strands.
Download Motor.csv (469.6 Kb)
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Title Assembly of motor components
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Description Agarose gel showing assembly of a surface connector, two folded squares and an unfolded square.
Download RotaryMotorAssemblySquaresGel.jpg (7.959 Kb)
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Title Polyacrylamide gel presented in Fig 4(d) of paper
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Description Polyacrylamide gel, showing the most important bands. From left to right: unfolded square of type B, folded squares of both types (B then A) and assembled motor - ‘before’ and ‘after’ operation.
Download PAGE_Fig4d.tif (51.05 Kb)
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Title AFM images acquired in response to reviewer comments
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Description AFM images of individual folded squares and assembled motors. The structures were extraordinarily difficult to image due to their small size. Consequently they adhered poorly to the substrate and were not well-resolved.
Download AFM.zip (793.4 Kb)
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When using this data, please cite the original publication:

Dunn KE, Leake MC, Wollman AJM, Trefzer MA, Johnson S, Tyrrell AM (2017) An experimental study of the putative mechanism of a synthetic autonomous rotary DNA nanomotor. Royal Society Open Science 4(3): 160767. http://dx.doi.org/10.1098/rsos.160767

Additionally, please cite the Dryad data package:

Dunn KE, Leake MC, Wollman AJM, Trefzer MA, Johnson S, Tyrrell AM (2017) Data from: An experimental study of the putative mechanism of a synthetic autonomous rotary DNA nanomotor. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.sj179
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