Data from: Predictability and irreversibility of genetic changes associated with flower color evolution in Penstemon barbatus

Wessinger CA, Rausher MD

Date Published: December 12, 2013

DOI: http://dx.doi.org/10.5061/dryad.sp800

 

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Title allQPCRdata
Downloaded 33 times
Description Includes all relevant relative expression data from the paper. In particular, F3'h, F3'5'h, Dfr, and Fls expression data. All data is relative to a single Penstemon neomexicanus floral cDNA sample and has been relativized to expression of elongation factor alpha.
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Title DFRenzymeData
Downloaded 43 times
Description Data from DFR in vitro enzyme assays - includes relative amounts of DHK, DHQ, and DHM converted by each species.
Download DFRenzymeData.xls (36.35 Kb)
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Title F2sAlleleSpecificExpr
Downloaded 28 times
Description Allele specific expression at F3'5'h in selected F2 individuals. The gene was amplified and cloned from cDNA. Then individual colonies were subjected to PCR and CAPS marker genotyping to determine the relative abundance of the two alleles in the cDNA sample.
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Title F3'5'hInVitroAssayData
Downloaded 46 times
Description F3'5'h in vitro enzyme assay data for Penstemon neomexicanus, P. barbatus, and our experimental constructs. DHQ production is considered an intermediate reaction product and was included in our measurement of "amount of DHK converted".
Download F3'5'hInVitroAssayData.xls (47.61 Kb)
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Title PyroseqData
Downloaded 40 times
Description Allele-specific expression data obtained through pyrosequencing. We compared differences in F3'5'h allelic expression in cDNA to gDNA for three F1 individuals
Download PyroseqData.xls (38.4 Kb)
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When using this data, please cite the original publication:

Wessinger CA, Rausher MD (2013) Predictability and irreversibility of genetic changes associated with flower color evolution in Penstemon barbatus. Evolution 68(4): 1058–1070. http://dx.doi.org/10.1111/evo.12340

Additionally, please cite the Dryad data package:

Wessinger CA, Rausher MD (2014) Data from: Predictability and irreversibility of genetic changes associated with flower color evolution in Penstemon barbatus. Dryad Digital Repository. http://dx.doi.org/10.5061/dryad.sp800
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