Data from: Changes in predator exposure, but not diet induce phenotypic plasticity in scorpion venom

Gangur AN, Smout M, Liddell MJ, Seymour JE, Wilson D, Northfield TD

Date Published: August 18, 2017

DOI: https://doi.org/10.5061/dryad.sq2g4

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Title First milking data
Downloaded 7 times
Description FPLC absorbance time-series output for the initial (before treatments, t=0) venom extraction. Each of the four treatments are presented on separate sheets. Each column is a different scorpion, values are absorbance at 280nm. The leftmost column specifies the volume eluted in mL (beginning at 5mL due to an initial 5 mL to prime the equipment).
Download First milking data.xlsx (7.870 Mb)
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Title Second milking data
Downloaded 8 times
Description FPLC absorbance time-series output for the second (after completion of treatments, t=1) venom extraction. Each of the four treatments are presented on separate sheets. Each column is a different scorpion, values are absorbance at 280nm. The leftmost column specifies the volume eluted in mL (beginning at 5mL due to an initial 5 mL to prime the equipment).
Download Second milking data.xlsx (6.977 Mb)
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Title Third milking data
Downloaded 6 times
Description FPLC absorbance time-series output for the third (3 weeks after the second venom extraction, t=2) venom extraction. Each of the four treatments are presented on separate sheets. Each column is a different scorpion, values are absorbance at 280nm. The leftmost column specifies the volume eluted in mL (beginning at 5mL due to an initial 5 mL to prime the equipment).
Download Third milking data.xlsx (7.591 Mb)
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Title xCELLigence output for vertebrate cell assays
Downloaded 5 times
Description Raw xCELLigence output measuring changes to cell index in response to each venom fraction and the whole venom mixture (20 µL). Cell response is a combination measure of changes in media conductivity or cell contact/toxicity, which varies as the cells deform in response to exposure to a chemical sample. The maximum drop value in 2 hours after venom addition was deemed the predator cell response. Each fraction was replicated with 3 cell wells.
Download xCELLigence output for vertebrate cell assays.xlsx (150.9 Kb)
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Title Cricket bioassay results
Downloaded 3 times
Description Caption: Results of invertebrate toxicity assays, evaluating toxin activity using the righting response of immature crickets to each venom fraction. 3 µL of one of the 14 chemical fractions was injected ventrally into the pronotum of an immature cricket, which was then rolled onto its back every 10 seconds 18 times for a total of 3 minutes. A compromised righting response was recorded when a cricket was unable to right itself within 60 seconds of being rolled onto its dorsal side. Each fraction was replicated with 10 crickets.
Download Cricket bioassay results.xlsx (21.21 Kb)
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When using this data, please cite the original publication:

Gangur AN, Smout M, Liddell MJ, Seymour JE, Wilson D, Northfield TD (2017) Changes in predator exposure, but not in diet, induce phenotypic plasticity in scorpion venom. Proceedings of the Royal Society B 284(1863): 20171364. https://doi.org/10.1098/rspb.2017.1364

Additionally, please cite the Dryad data package:

Gangur AN, Smout M, Liddell MJ, Seymour JE, Wilson D, Northfield TD (2017) Data from: Changes in predator exposure, but not diet induce phenotypic plasticity in scorpion venom. Dryad Digital Repository. https://doi.org/10.5061/dryad.sq2g4
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