Tomato fruit shape varies significantly in the cultivated germplasm. To a large extent, this variation can be explained by four genes including OVATE. While most varieties with the OVATE mutation bear elongated fruits, some accessions carry round fruit suggesting the existence of suppressors of OVATE in the germplasm. We developed three intraspecific F_2 populations with parents that carried the OVATE mutation but differed in fruit shape. We used a Bulk Segregant Analysis approach and genotyped the extreme classes using a high-throughput genotyping platform, the SolCAP Infinium Assay. The analyses revealed segregation at two QTL, sov1 and sov2. These loci were confirmed by genotyping and QTL analyses of the entire population. More precise location of those loci using progeny testing confirmed that sov1 on chromosome 10 controlled obovoid and elongated shape whereas sov2 on chromosome 11 controlled mainly elongated fruit shape. Both loci were located in intervals of less than 2.4 Mb on their respective chromosomes.
Phenotype and molecular data for each F2 population
This file contains phenotype and molecular marker data from three F2 populations. Each spreadsheet corresponds to one population. 09S95, F2 T1693 x YP. 09S96, F2 GBL x YP. 09S97, F2 SM x GBL. Abbreviations: YP, cv Yellow Pear. GBL, cv Gold Ball Livingston. SM, cv San Marzano. T1693, cv T1693.
Raw genotype data extracted from Genome studio Software
This file contains the genotype scores for 7720 SNPs generated with the SolCAP Illumina Infinium Assay for 40 samples. The samples are the parental genotypes: YP, cv Yellow Pear. GBL, cv Gold Ball Livingston. SM, cv San Marzano. T1693, cv T1693. B95A, bulk with the 15 most round genotypes in 09S95 population. B95B, bulk with the 15 most elongated genotypes in 09S95 population. B96A, bulk with the 15 most round genotypes in 09S96 population. B96B, bulk with the 15 most elongated genotypes in 09S96 population. B97A, bulk with the 15 most round genotypes in 09S97 population. B97B, bulk with the 15 most elongated genotypes in 09S97 population. Also the 15 most round and 15 most elongated genotypes in the F2 GBL x YP (09S96) are shown.
T-test result of each recombinant F2-F3 family
This file contains phenotype and molecular marker data used for progeny test in F2-F3 recombinant families. The t-test result for fruit shape index in each recombinant family also is shown.
Parents_and_F1_crosses
This zipped file contains longitudinal fruit images of parental genotypes and F1 crosses. YP, cv Yellow Pear. GBL, cv Gold Ball Livingston. SM, cv San Marzano. T1693, cv T1693. 09S92, F1 (T1693 x YP). 09S93, F1 (GBL x YP). 09S94, F1 (SM x GBL).
09S95
This zipped file contains longitudinal fruit images of each F2 T1693 x YP plant. Population 09S95
09S96
This zipped file contains longitudinal fruit images of each F2 GBL x YP plant. Population 09S96
09S97
This zipped file contains longitudinal fruit images of each F2 SM x GBL plant. Population 09S97
Images _each recombinant family
This zipped files contains longitudinal fruit images of each F2-F3 recombinant family